ArticleJournal of biomedical optics2022
Multiresolution nondestructive 3D pathology of whole lymph nodes for breast cancer staging.
Article in Journal of biomedical optics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 10 citations in OpenAlex.
- Optical Tissue Clearing and Small-Molecule Labeling of Paraffin-Embedded Breast Cancer and Axillary Lymph Node Human Tissue Samples.Bio-protocol · 2026Article
- Deep-learning triage of three-dimensional pathology datasets for comprehensive and efficient pathologist assessments.Nature biomedical engineering · 2026Article
- Volumetric Cyclic Immunofluorescence for 3D Spatial Profiling of Immune Structures in Human FFPE Tissue.bioRxiv : the preprint server for biology · 2026Article
- Confocal-Compatible Workflow for Sectioning, Staining, and Imaging Serial Vibratome Sections for 3D Anatomical Reconstruction of the Lymph Node.bioRxiv : the preprint server for biology · 2025Article
- Quantitative and comparative assessment of dyes and protocols for rapid ex vivo microscopy of fresh tissues.Scientific reports · 2024Article
- Mechanically sheared axially swept light-sheet microscopy.Biomedical optics express · 2024Article
- descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.Nature communications · 2024Article
- Article
- An end-to-end workflow for nondestructive 3D pathology.Nature protocols · 2024Review
- Blueprints from plane to space: outlook of next-generation three-dimensional histopathology.Cancer science · 2024Review
- Structured illumination microscopy for cancer identification in diagnostic breast biopsies.PloS one · 2024Article
- An end-to-end workflow for non-destructive 3D pathology.bioRxiv : the preprint server for biology · 2023Article
- Nondestructive 3D Pathology with Light-Sheet Fluorescence Microscopy for Translational Research and Clinical Assays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
significanceFor breast cancer patients, the extent of regional lymph node (LN) metastasis influences the decision to remove all axillary LNs. Metastases are currently identified and classified with visual analysis of a few thin tissue sections with conventional histology that may underrepresent the extent of metastases.
aimWe sought to enable nondestructive three-dimensional (3D) pathology of human axillary LNs and to develop a practical workflow for LN staging with our method. We also sought to evaluate whether 3D pathology improves staging accuracy in comparison to two-dimensional (2D) histology. APPROACH: We developed a method to fluorescently stain and optically clear LN specimens for comprehensive imaging with multiresolution open-top light-sheet microscopy. We present an efficient imaging and data-processing workflow for rapid evaluation of H&E-like datasets in 3D, with low-resolution screening to identify potential metastases followed by high-resolution localized imaging to confirm malignancy.
resultsWe simulate LN staging with 3D and 2D pathology datasets from 10 metastatic nodes, showing that 2D pathology consistently underestimates metastasis size, including instances in which 3D pathology would lead to upstaging of the metastasis with important implications on clinical treatment.
conclusionsOur 3D pathology method may improve clinical management for breast cancer patients by improving staging accuracy of LN metastases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.