ArticleParasitology research2022
Molecular identification of Theileria species in naturally infected sheep using nested PCR-RFLP.
Article in Parasitology research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 14 citations in OpenAlex.
- Prevalence and molecular detection ofParasite epidemiology and control · 2026Article
- Genetic diversity and molecular identification of sheep infected withOpen veterinary journal · 2026Article
- Molecular Evidence of Ovine Theileriosis in Selected Areas of Qinghai Province.Veterinary sciences · 2026Article
- Development and application of a TaqMan probe-based pentaplex qPCR for the rapid detection and differential identification of five pathogens.Frontiers in microbiology · 2026Article
- Theileria lestoquardi and malignant theileriosis in small ruminants: an updated review.Tropical animal health and production · 2025Review
- Molecular detection and assessment of risk factors for Theileria lestoquardi in sheep from Balochistan, Pakistan.Parasitology research · 2023Article
- First morphometric and molecular characterization of Fasciola spp. in Northwest Tunisia.Parasitology research · 2023Article
- First Molecular Confirmed Outbreak of Malignant Ovine Theileriosis in Sheep from North India.Acta parasitologica · 2023Article
- Molecular identification and genetic characterization of Theileria and Anaplasma infection in sheep and goat of North Gujarat, India.Parasitology research · 2023Article
- Small Ruminant Piroplasmosis: High Prevalence ofPathogens (Basel, Switzerland) · 2023Article
- Article
- Anaemia in Sheep Caused byAnimals : an open access journal from MDPI · 2022Review
- Molecular Detection and Differentiation of Different Theileria Species in Naturally Infected Goats Using Nested PCR-RFLP: A First Report from Northern India.Acta parasitologica · 2022Article
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4 authors at 2 institutions in 1 country.
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Abstract
Ovine theileriosis is an important tick-borne haemoprotozoan disease of sheep in tropical and subtropical regions, causing severe productivity and economic loss. There is a paucity of information related to molecular studies of ovine theileriosis from India. The present study identified different Theileria spp. in naturally infected sheep using nested PCR-restriction fragment length polymorphism (nPCR-RFLP). Blood samples and ticks were collected from 204 sheep in different agro-climatic zones of Haryana state, India, during the tick active season. Microscopic examination of thin blood smears revealed 33.3% (68/204) infections with Theileria spp., while 44.6% (91/204) of blood samples were positive by nPCR assay. Different Theileria spp. were identified based upon RFLP patterns using four restriction enzymes: Hpa II, Bsh 1285I, Hae II and Rsa I. Out of 91 positive samples, 50.5% (46/91), 23.08% (21/91), 11% (10/91) and 2.2% (2/91) were positive for T. ovis, T. lestoquardi, T. luwenshuni (Theileria sp. China 1/Theileria sp. China) and T. annulata, respectively. Mixed infection was detected in 13.2% (12/91) of cases. Based upon HpaII enzymatic digestion pattern, two samples with T. lestoquardi and T. annulata, nine samples with T. lestoquardi and T. ovis and one sample with T. ovis and T. annulata were detected. The presence of these Theileria spp. was further confirmed by sequence analysis. The majority of ticks collected from sheep were identified as Rhipicephalus spp. followed by Hyalomma anatolicum and Hemaphysalis spp. The present investigation depicts the first comprehensive molecular report of naturally infected sheep with T. ovis, T. lestoquardi, T. annulata and T. luwenshuni from northern India.
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