ReviewNature protocols2022
In vivo and ex vivo electrophysiological study of the mouse heart to characterize the cardiac conduction system, including atrial and ventricular vulnerability.
Review in Nature protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 17 citations in OpenAlex.
- A filamentary soft robotic probe for multimodal in utero monitoring of fetal health.Nature biomedical engineering · 2026Article
- HCN4 gain-of-function mutation increases intrinsic heart rate and limits maladaptive remodeling under pressure overload.Frontiers in pharmacology · 2026Article
- The isolated, perfused working heart preparation of the mouse-Advantages and pitfalls.Acta physiologica (Oxford, England) · 2025Review
- Efficacy of Quality and Quantity media-cultured mononuclear cells for promoting peripheral nerve regeneration in mouse model.PloS one · 2025Article
- Article
- [Intrinsic steady-state pattern of mouse cardiac electrophysiology: analysis using a characterized quantitative electrocardiogram strategy].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2024Article
- Isolated Perfused Hearts for Cardiovascular Research: An Old Dog with New Tricks.Journal of cardiovascular translational research · 2024Review
- A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles.Cell research · 2024Article
- A comprehensive protocol combining in vivo and ex vivo electrophysiological experiments in an arrhythmogenic animal model.American journal of physiology. Heart and circulatory physiology · 2024Article
- Intracardiac electrophysiology to characterize susceptibility to ventricular arrhythmias in murine models.Frontiers in physiology · 2024Article
- KairoSight-3.0: A validated optical mapping software to characterize cardiac electrophysiology, excitation-contraction coupling, and alternans.Journal of molecular and cellular cardiology plus · 2023Article
- Organ-on-chip systems as a model for nanomedicine.Nanoscale · 2023Review
- Overview of programmed electrical stimulation to assess atrial fibrillation susceptibility in mice.Frontiers in physiology · 2023Review
- Guidelines for assessment of cardiac electrophysiology and arrhythmias in small animals.American journal of physiology. Heart and circulatory physiology · 2022Review
- Paradigm shift: new concepts for HCN4 function in cardiac pacemaking.Pflugers Archiv : European journal of physiology · 2022Review
- Demographic and Methodological Heterogeneity in Electrocardiogram Signals From Guinea Pigs.Frontiers in physiology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mouse is a common and cost-effective animal model for basic research, and the number of genetically engineered mouse models with cardiac phenotype is increasing. In vivo electrophysiological study in mice is similar to that performed in humans. It is indispensable for acquiring intracardiac electrocardiogram recordings and determining baseline cardiac cycle intervals. Furthermore, the use of programmed electrical stimulation enables determination of parameters such as sinoatrial conduction time, sinus node recovery time, atrioventricular-nodal conduction properties, Wenckebach periodicity, refractory periods and arrhythmia vulnerability. This protocol describes specific procedures for determining these parameters that were adapted from analogous human protocols for use in mice. We include details of ex vivo electrophysiological study, which provides detailed insights into intrinsic cardiac electrophysiology without external influences from humoral and neural factors. In addition, we describe a heart preparation with intact innervation by the vagus nerve that can be used as an ex vivo model for vagal control of the cardiac conduction system. Data acquisition for in vivo and ex vivo electrophysiological study takes ~1 h per mouse, depending on the number of stimulation protocols applied during the procedure. The technique yields highly reliable results and can be used for phenotyping of cardiac disease models, elucidating disease mechanisms and confirming functional improvements in gene therapy approaches as well as for drug and toxicity testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.