ArticleNature communications2022
New recognition specificity in a plant immune receptor by molecular engineering of its integrated domain.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Harnessing Natural Diversity and Rational Design for Enhanced Plant Immunity.Plant, cell & environment · 2026Review
- Engineering plant tandem kinase immune receptors expands effector recognition profiles.Science advances · 2026Article
- Molecular Bases and Genetic Design of Rice Disease Resistance for Optimized Yield and Sustainable Agriculture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Mechanisms and balanced regulation of plant immunity.Nature reviews. Molecular cell biology · 2026Review
- The Rice-Xoc Arms Race: Molecular Mechanisms and Genomic Strategies Against Bacterial Leaf Streak.Rice (New York, N.Y.) · 2026Review
- An HMA-like integrated domain in the wheat tandem kinase WTK4 recognises an RNase-like pathogen effector.Nature communications · 2026Article
- Two folds, many faces: The Magnaporthe oryzae MAX effector AVR-Pia targets novel rice HMA domain-containing proteins.PLoS pathogens · 2026Article
- Structure-informed engineering of plant-microbe interactions.The Plant journal : for cell and molecular biology · 2026Review
- Article
- Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance.The Plant cell · 2026Article
- Dual recognition of structurally unrelated mildew effectors underlies the broad-spectrum resistance of Pm3e in wheat.Nature communications · 2026Article
- Plant immune inducers from fundamental research to applications: Past achievements, current challenges, and future perspectives.Plant communications · 2026Review
- Mismatch screening in Nicotiana benthamiana to explore Pik-1/Pik-2 paired NLR platforms for receptor engineering.The New phytologist · 2026Article
- Unraveling plant immunity: from pathogen perception to resistance engineering.Science China. Life sciences · 2026Review
- Precision Editing of NLRS Improves Effector Recognition for Enhanced Disease Resistance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- From gene-for-gene to NLRome-for-effectorome: decoding the dynamic interplay between phytopathogenic effector networks and plant immune systems.FEMS microbiology reviews · 2026Review
- TheProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structural Insights Into the Role of RxLR Effectors in the Arms Race Between Oomycetes and Plants.Molecular plant pathology · 2025Review
- Article
- Utilizing plant synthetic biology to accelerate plant-microbe interactions research.Biodesign research · 2025Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant nucleotide-binding and leucine-rich repeat domain proteins (NLRs) are immune sensors that recognize pathogen effectors. Here, we show that molecular engineering of the integrated decoy domain (ID) of an NLR can extend its recognition spectrum to a new effector. We relied for this on detailed knowledge on the recognition of the Magnaporthe oryzae effectors AVR-PikD, AVR-Pia, and AVR1-CO39 by, respectively, the rice NLRs Pikp-1 and RGA5. Both receptors detect their effectors through physical binding to their HMA (Heavy Metal-Associated) IDs. By introducing into RGA5_HMA the AVR-PikD binding residues of Pikp-1_HMA, we create a high-affinity binding surface for this effector. RGA5 variants carrying this engineered binding surface perceive the new ligand, AVR-PikD, and still recognize AVR-Pia and AVR1-CO39 in the model plant N. benthamiana. However, they do not confer extended disease resistance specificity against M. oryzae in transgenic rice plants. Altogether, our study provides a proof of concept for the design of new effector recognition specificities in NLRs through molecular engineering of IDs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.