Evidence map›Paper›PMID 35314662›Full record

ArticleMethods and protocols2022

Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples.

Francesco Saverio Tarantini, Siyu Wu, Harry Jenkins, Ana Tellechea Lopez, Hannah Tomlin, Ralph Hyde, Katarzyna Lis-Slimak, Jamie Louise Thompson, Sara Pijuan-Galitó, Danielle Scales and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods and protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 1 institution in 1 country.

Francesco Saverio TarantiniAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Siyu WuAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Harry JenkinsAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Ana Tellechea LopezAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Hannah TomlinAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Ralph HydeAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Katarzyna Lis-SlimakAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Jamie Louise ThompsonAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Sara Pijuan-GalitóAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.ORCID 0000-0002-7398-9441
Danielle ScalesAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Kazuyo KanekoAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Jayasree DeyAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Emily ParkAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Jack HillAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
I-Ning LeeAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Lara DoolanAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Asta Arendt-TranholmAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Chris DenningAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.
Claire SeedhouseAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.ORCID 0000-0003-2280-4045
Andrew V BenestAsymptomatic Testing Service, Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham NG7 2RD, UK.ORCID 0000-0002-8225-5159
University of Nottingham · GB

Funding

British Heart Foundation PG/18/31/33759
6 · The paper itself

Abstract

Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal 'testing fatigue' and reduced confidence in test results. Consequently, asymptomatic individuals have developed reluctance towards repeat testing, which remains the best way to monitor COVID-19 cases in the wider population. On the other hand, saliva-based PCR, a non-invasive, highly sensitive, and accurate test suitable for everyone, is gaining momentum as a straightforward and reliable means of detecting SARS-CoV-2 in symptomatic and asymptomatic individuals. Here, we provide an itemised list of the equipment and reagents involved in the process of sample submission, inactivation and analysis, as well as a detailed description of how each of these steps is performed.

Indexed as

COVID-19qPCRsaliva

Identifiers

PMID35314662
PMCPMC8938789
OpenAlexW4220824780

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.