ArticleReproductive biology and endocrinology : RB&E2022
Comparative analyses in transcriptome of human granulosa cells and follicular fluid micro-environment between poor ovarian responders with conventional controlled ovarian or mild ovarian stimulations.
Article in Reproductive biology and endocrinology : RB&E, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.
- Efficacy of intraovarian autologous platelet-rich plasma in women with poor ovarian reserve or ovarian insufficiency: a meta-analysis of randomized controlled trials.Frontiers in endocrinology · 2026Pooled it
- Another round, another chance: oocyte developmental competence and outcomes in second IVF attempts-the earlier, the better.Journal of assisted reproduction and genetics · 2026Observational
- Phenotype-driven protocol switching is associated with improved ART outcomes under constant gonadotropin dosage: a self-controlled analysis of 4,632 cycles.Frontiers in endocrinology · 2026Article
- Assessment indicators of ovarian response during controlled ovarian stimulation: influencing factors and clinical value.Frontiers in endocrinology · 2026Review
- Proximity extension assay revealed novel inflammatory biomarkers for follicular development and ovarian function: a prospective controlled study combining serum and follicular fluid.Frontiers in endocrinology · 2025Article
- Gene expression analysis of ovarian follicles and stromal cells in girls with Turner syndrome.Molecular human reproduction · 2024Article
- Sestrin1, 2, and 3 are dispensable for female fertility in mice.Journal of ovarian research · 2024Article
- Effect of bovine and human follicular fluid on semen quality of fresh and frozen-thawed semen in dual purpose Simmental (Fleckvieh) bulls: a new method of adding follicular fluid to bull semen.Iranian journal of veterinary research · 2024Article
- Biochemical profiling of the follicular environment to predict oocyte competence in cattle.PloS one · 2024Article
- Fluid biopsies forMedicine internationalReview
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundBoth mild and conventional controlled ovarian stimulation are the frequently used protocols for poor ovarian responders. However, there are some debates about which treatment is better. Moreover, little is known about the follicular physiology after the two ovarian stimulation protocols. This study was intended to investigate the features in granulosa cells and follicular fluid micro-environment after the two different ovarian stimulation protocols in poor responders.
methodsGranulosa cells RNA were sequenced using Illumina Hiseq technology. Specific differently expressed genes and proteins were verified by real-time quantitative PCR and Western blot analysis. Moreover, hormone and cytokine concentrations in the follicular fluid were measured by electrochemiluminescence immunoassay and enzyme-linked immunoabsorbent assay. The correlation between the results of molecular experiments and the laboratory outcomes were analyzed by Spearman correlation analysis.
resultsThe differentially expressed genes between the two groups were involved in 4 signaling pathways related to the follicular development; three proteins pertinent to the TGF-β signaling pathway were expressed differently in granulosa cells between the two, and the constituents in the follicular fluid were also different. Further, a correlation between the TGF-β signaling pathway and the good-quality embryo was observed.
conclusionsThe present study made a comparison for the first time in the transcriptome of human granulosa cells and the follicular fluid micro-environment between poor responders with the conventional controlled ovarian stimulation or the mild ovarian stimulation, showing that the TGF-β signaling pathway may correlate with the good-quality of embryos in the mild group, which may be instrumental to the choice of optimal management for IVF patients.
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Registered trials
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