Evidence map›Paper›PMID 35313831›Full record

ArticleBMC cancer2022

Expression of the preadipocyte marker ZFP423 is dysregulated between well-differentiated and dedifferentiated liposarcoma.

Thanh N Dang, Rafael P Tiongco, Loren M Brown, Jessica L Taylor, John M Lyons, Frank H Lau, Z Elizabeth Floyd

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.7field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Thanh N DangPennington Biomedical Research Center, Baton Rouge, Louisiana, 70808, USA.
Rafael P TiongcoTulane University School of Medicine, New Orleans, Louisiana, 70118, USA.
Loren M BrownDepartment of Surgery, Louisiana State University Health Science Center, New Orleans, Louisiana, 70112, USA.
Jessica L TaylorPennington Biomedical Research Center, Baton Rouge, Louisiana, 70808, USA.
John M LyonsOur Lady of the Lake Medical Center, Baton Rouge, Louisiana, 70808, USA.
Frank H LauDepartment of Surgery, Louisiana State University Health Science Center, New Orleans, Louisiana, 70112, USA. flau@lsuhsc.edu.
Z Elizabeth FloydPennington Biomedical Research Center, Baton Rouge, Louisiana, 70808, USA. elizabeth.floyd@pbrc.edu.
Pennington Biomedical Research Center · USLouisiana State University Health Sciences Center New Orleans · USOur Lady of the Lake Regional Medical Center · USTulane University · US

Funding

Research BaseP30DK072476 · NIDDK · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · PI Sujoy Ghosh · 2005 to 2026
$26.5M
NIDDK NIH HHS P30 DK072476
6 · The paper itself

Abstract

backgroundWell-differentiated and dedifferentiated liposarcomas are rare soft tissue tumors originating in adipose tissue that share genetic abnormalities but have significantly different metastatic potential. Dedifferentiated liposarcoma (DDLPS) is highly aggressive and has an overall 5-year survival rate of 30% as compared to 90% for well-differentiated liposarcoma (WDLPS). This discrepancy may be connected to their potential to form adipocytes, where WDLPS is adipogenic but DDLPS is adipogenic-impaired. Normal adipogenesis requires Zinc Finger Protein 423 (ZFP423), a transcriptional coregulator of Perixosome Proliferator Activated Receptor gamma (PPARG2) mRNA expression that defines committed preadipocytes. Expression of ZFP423 in preadipocytes is promoted by Seven-In-Absentia Homolog 2 (SIAH2)-mediated degradation of Zinc Finger Protein 521 (ZFP521). This study investigated the potential role of ZFP423, SIAH2 and ZFP521 in the adipogenic potential of WDLPS and DDLPS.

methodsHuman WDLPS and DDLPS fresh and paraffin-embedded tissues were used to assess the gene and protein expression of proadipogenic regulators. In parallel, normal adipose tissue stromal cells along with WDLPS and DDLPS cell lines were cultured, genetically modified, and induced to undergo adipogenesis in vitro.

resultsImpaired adipogenic potential in DDLPS was associated with reduced ZFP423 protein levels in parallel with reduced PPARG2 expression, potentially involving regulation of ZFP521. SIAH2 protein levels did not define a clear distinction related to adipogenesis in these liposarcomas. However, in primary tumor specimens, SIAH2 mRNA was consistently upregulated in DDLPS compared to WDLPS when assayed by fluorescence in situ hybridization or real-time PCR.

conclusionsThese data provide novel insights into ZFP423 expression in adipogenic regulation between WDLPS and DDLPS adipocytic tumor development. The data also introduces SIAH2 mRNA levels as a possible molecular marker to distinguish between WDLPS and DDLPS.

Indexed as

DNA-Binding ProteinsAdipogenesisBiomarkers, TumorCell Line, TumorHumansLiposarcomaNuclear ProteinsSeven in Absentia ProteinsSoft Tissue NeoplasmsUbiquitin-Protein LigasesZinc FingersBiomarkers, TumorDNA-Binding ProteinsNuclear ProteinsSeven in Absentia ProteinsUbiquitin-Protein Ligaseszinc finger protein 521, humanAdipogenesisLiposarcomaPPARgammaSIAH2ZFP423ZFP521

Identifiers

PMID35313831
PMCPMC8939188
OpenAlexW4220662348

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.