Evidence map›Paper›PMID 35313798›Full record

ArticleGenetics, selection, evolution : GSE2022

Divergent selection for natural antibodies in poultry in the presence of a major gene.

Henk Bovenhuis, Tom V L Berghof, Marleen H P W Visker, Joop A J Arts, Jeroen Visscher, Jan J van der Poel, Henk K Parmentier

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genetics, selection, evolution : GSE, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Henk BovenhuisAnimal Breeding and Genomics Centre, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands. henk.bovenhuis@wur.nl.ORCID http://orcid.org/0000-0002-9074-5334
Tom V L BerghofAnimal Breeding and Genomics Centre, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
Marleen H P W ViskerAnimal Breeding and Genomics Centre, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
Joop A J ArtsAdaptation Physiology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
Jeroen VisscherHendrix Genetics Research Technology & Service B.V, P.O. Box 114, 5830 AC, Boxmeer, The Netherlands.
Jan J van der PoelAnimal Breeding and Genomics Centre, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
Henk K ParmentierAdaptation Physiology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
Wageningen University & Research · NLHendrix Genetics (Netherlands) · NLTechnical University of Munich · DE

Funding

Stichting voor de Technische Wetenschappen 12208
6 · The paper itself

Abstract

backgroundNatural antibodies (NAb) are antibodies that are present in a healthy individual without requiring previous exposure to an exogenous antigen. Selection for high NAb levels might contribute to improved general disease resistance. Our aim was to analyse the genetic background of NAb based on a divergent selection experiment in poultry, and in particular the effect of a polymorphism in the TLR1A gene.

methodsThe study population consisted of a base population from a commercial pure-bred elite white leghorn layer line and seven generations of birds from a High and Low selection line. Birds were selected for total KLH-binding NAb titer (IgTotal). An enzyme-linked immunosorbent assay was performed to determine NAb titers in blood plasma for IgTotal and the antibody isotypes IgM and IgG. NAb titers were available for 10,878 birds. Genotypes for a polymorphism in TLR1A were determined for chickens in generations 5, 6 and 7. The data were analysed using mixed linear animal models.

resultsThe heritability estimate for IgM was 0.30 and higher than that for IgG and IgTotal (0.12). Maternal environmental effects explained 2 to 3% of the phenotypic variation in NAb. Selection for IgTotal resulted in a genetic difference between the High and Low line of 2.4 titer points (5.1 genetic standard deviation) in generation 7. For IgM, the selection response was asymmetrical and higher in the Low than the High line. The frequency of the TLR1A C allele was 0.45 in the base population and 0.66 and 0.04 in generation 7 of the High and Low line, respectively. The TLR1A polymorphism had large and significant effects on IgTotal and IgM. Estimated genotypic effects suggest full dominance of the TLR1A C allele. Significant TLR1A by generation interactions were detected for IgM and IgTotal.

conclusionsThe effect of a polymorphism in the TLR1A gene on IgTotal and IgM NAb was confirmed. Furthermore, we provide experimental verification of changes in allele frequencies at a major gene with dominant gene action on a quantitative trait that is subjected to mass selection. TLR1A by generation interactions indicate sensitivity to environmental factors.

Indexed as

ChickensPoultryAnimalsBreedingHumansImmunoglobulin GImmunoglobulin MImmunoglobulin GImmunoglobulin M

Identifiers

PMID35313798
PMCPMC8939063
OpenAlexW4220836774

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.