ArticleVeterinary clinical pathology2022
Analytical validation of a novel point-of-care immunoassay for canine N-terminal pro-brain natriuretic peptide analysis.
Article in Veterinary clinical pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Pre-anaesthetic risk assessment and management of dogs with myxomatous mitral valve disease: a spectrum of care narrative review.The Journal of small animal practice · 2026Review
- Effects of a resveratrol-containing nutraceutical on cardiac biomarkers, echocardiographic parameters, and oxidative stress in dogs with naturally occurring cardiac disease.Frontiers in veterinary science · 2026Article
- Diagnostic performance of Vcheck NT-proBNP and cardiac troponin I assays for detection of dilated cardiomyopathy in Doberman Pinschers.Frontiers in veterinary science · 2026Article
- Analytical Validation of a Novel Point-Of-Care Quantitative Immunoassay for Feline N-Terminal Pro-Brain Natriuretic Peptide.Veterinary clinical pathology · 2025Article
- Pulmonary hypertension and NT-proBNP dynamics during the course of adulticide treatment in dogs naturally infected by Dirofilaria immitis.Parasites & vectors · 2025Article
- Article
- Domestic dogs maintain clinical, nutritional, and hematological health outcomes when fed a commercial plant-based diet for a year.PloS one · 2024Article
- Usefulness of NT-proBNP in dogs with heartworm: could this biomarker be useful to evaluate pulmonary hypertension?Parasites & vectors · 2023Article
- Analytical validation of a novel point-of-care immunoassay for canine N-terminal pro-brain natriuretic peptide analysis.Veterinary clinical pathology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundN-terminal pro-brain natriuretic peptide (NT-proBNP) is a widely used point-of-care (POC) cardiac biomarker in human medicine. Canine NT-proBNP is used less in veterinary medicine, possibly due to the lack of a POC canine NT-proBNP assay resulting in temporal delay, increased degradation in transport, and high reported variability in the available assay. A new quantitative POC analyzer allows fast, onsite measurement of NT-proBNP, minimizing preanalytical error and reducing variability.
objectiveWe aimed to analytically validate an NT-proBNP assay (Vcheck) according to American Society of Veterinary Clinical Pathology (ASVCP) and Clinical Laboratory Improvement Amendments (CLIA) specifications.
methodsArchived and prospective plasma and serum samples were collected from male and female, client-owned dogs of various breeds with cardiac abnormalities (n = 81) and a healthy control population (n = 225). Precision, accuracy, analytical sensitivity, and specificity, and other statistical analyses were performed.
resultsImprecision was considered acceptable with a coefficient of variation ranging from 9% at 4000 pmol/L to 20% at 600 pmol/L. The lower limit of quantitation was 650 pmol/L based on repetitive measures evaluation. Comparison of the Vcheck assay with the Cardiopet NT-proBNP assay revealed an excellent correlation with minimal bias when preanalytical factors were controlled. Significant degradation of NT-proBNP occurred when current methods were used at refrigerated and room temperatures, which could change diagnostic and prognostic decision-making. Age-partitioned reference intervals have high reference values of 750 pmol/L and 1280 pmol/L for juvenile and adult dogs, respectively.
conclusionsThe Vcheck NT-ProBNP assay provides analytically acceptable results. Onsite testing can minimize variability related to preanalytical error and provide clinically useful contemporaneous results. Samples should be centrifuged immediately and analyzed within 2 hours of collection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.