Evidence map›Paper›PMID 35311103›Full record

ArticleFrontiers in oncology2022

Expression of GPR68, an Acid-Sensing Orphan G Protein-Coupled Receptor, in Breast Cancer.

Noha Mousaad Elemam, Rana A Youness, Amal Hussein, Israa Shihab, Nada M Yakout, Yasmine Nagy Elwany, Tamer M Manie, Iman M Talaat, Azzam A Maghazachi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in oncology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. pH-sensing GPR68 inhibits vascular smooth muscle cell proliferation through Rap1A.American journal of physiology. Heart and circulatory physiology · 2024
    Article
  7. Review
  8. Puzzling out the role ofNon-coding RNA research · 2024
    Review
  9. Recent advances in acid sensing by G protein coupled receptors.Pflugers Archiv : European journal of physiology · 2024
    Review
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Noha Mousaad ElemamClinical Sciences Department, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
Rana A YounessBiology and Biochemistry Department, School of Life and Medical Sciences, University of Hertfordshire Hosted by Global Academic Foundation, Cairo, Egypt.
Amal HusseinDepartment of Family and Community Medicine and Behavioral Sciences, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
Israa ShihabClinical Sciences Department, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
Nada M YakoutPathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Yasmine Nagy ElwanyClinical Oncology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Tamer M ManieDepartment of Breast Surgery, National Cancer Institute, Cairo University, Cairo, Egypt.
Iman M TalaatClinical Sciences Department, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
Azzam A MaghazachiClinical Sciences Department, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
University of Sharjah · AEAlexandria University · EGCairo University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Breast cancer (BC) is the most diagnosed cancer and the leading cause of global cancer incidence in 2020. It is quite known that highly invasive cancers have disrupted metabolism that leads to the creation of an acidic tumor microenvironment. Among the proton-sensing G protein-coupled receptors is GPR68. In this study, we aimed to explore the expression pattern of GPR68 in tissues from BC patients as well as different BC cell lines.

methodsIn-silico tools were used to assess the expression of GPR68 in BC patients. The expression pattern was validated in fresh and paraffin-embedded sections of BC patients using qPCR and immunohistochemistry (IHC), respectively. Also, in-silico tools investigated GPR68 expression in different BC cell lines. Validation of GPR68 expression was performed using qPCR and immunofluorescence techniques in four different BC cell lines (MCF-7, MDA-MB-231, BT-549 and SkBr3).

resultsGPR68 expression was found to be significantly increased in BC patients using the in-silico tools and validation using qPCR and IHC. Upon classification according to the molecular subtypes, the luminal subtype showed the highest GPR68 expression followed by triple-negative and Her2-enriched cells. However, upon validation in the recruited cohort, the triple-negative molecular subtype of BC patients showed the highest GPR68 expression. Also, in-silico and validation data revealed that the triple-negative breast cancer cell line MDA-MB-231 showed the highest expression of GPR68.

conclusionTherefore, this study highlights the potential utilization of GPR68 as a possible diagnostic and/or prognostic marker in BC.

Indexed as

acidosisbreast cancerGPR68triple-negative breast cancertumor microenvironment

Identifiers

PMID35311103
PMCPMC8930915
OpenAlexW4214886911

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.