ArticleFrontiers in bioengineering and biotechnology2022
The Synthesis of (Magnetic) Crosslinked Enzyme Aggregates With Laccase, Cellulase, β-Galactosidase and Transglutaminase.
Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Characterization of crude cellulase produced by Bacillus licheniformis OE-1 from the Diyadin hot springs, Ağrı Province.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
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- Synthesis and characterization of ZrFeNanoscale advances · 2026Article
- Catalytically active inclusion bodies of Bacillus clausii laccase protein recombinantly produced in E. coli for dye decolorization.Microbial cell factories · 2026Article
- Magnetic Bifunctional Ru-Enzyme Catalyst Allows for Sustainable Conversion of Cellulose Derivative to D-Sorbitol.Nanomaterials (Basel, Switzerland) · 2025Article
- Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization.Engineering in life sciences · 2025Review
- Article
- Natural Foraging Selection and Gut Microecology of Two Subterranean Rodents from the Eurasian Steppe in China.Animals : an open access journal from MDPI · 2024Article
- Advancements in enzyme immobilization on magnetic nanomaterials: toward sustainable industrial applications.RSC advances · 2024Review
- Transglutaminase in Foods and Biotechnology.International journal of molecular sciences · 2023Review
- Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing.Nanomaterials (Basel, Switzerland) · 2022Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immobilized enzymes have important aspects due to the fact that they possess higher stability, have the possibility to be easily removed from the reaction mixture, and are much easier to use when compared to free enzymes. In this research, the enzymes laccase, cellulase, β-galactosidase (β-gal), and transglutaminase (TGM) were immobilized by two different methods: crosslinked enzyme aggregates (CLEAs) and magnetic crosslinked enzyme aggregates (mCLEAs). The processes for CLEAs and mCLEAs preparation with different enzymes have been optimized, where the aim was to achieve the highest possible relative activity of the immobilized enzyme. The optimal conditions of the synthesis of CLEAs in mCLEAs are described, thus emphasizing the difference between the two types of immobilization based on different enzymes. This comparative study, which represents the synthesis of crosslinked enzyme aggregates using different enzymes, has not been performed so far. Moreover, the obtained activity of CLEAs and mCLEAs is presented, which is important for further use in different biocatalytic processes. Specifically, of a higher importance is the selection of enzymes involved in immobilization, as they belong to the three different most applicable enzymes (oxidoreductases, hydrolases, and transferases). The study confirmed that the resulting activity of the immobilized enzyme and the optimization of enzyme immobilization depended on the type of the enzyme. Moreover, the prepared CLEAs and mCLEAs were exposed to the supercritical carbon dioxide (scCO
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