ArticleChemical science2022
HydroFlipper membrane tension probes: imaging membrane hydration and mechanical compression simultaneously in living cells.
Article in Chemical science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Plasma membrane order maps functional diversity in immune cells.Nature chemical biology · 2026Article
- Chemical fluorophores for fluorescence lifetime imaging.Chemical Society reviews · 2026Review
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- Membrane Fusion-Based Drug Delivery Liposomes Transiently Modify the Material Properties of Synthetic and Biological Membranes.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
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- Redefining Molecular Probes for Monitoring Subcellular Environment: A Perspective.Analytical chemistry · 2024Review
- Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes.Nature protocols · 2024Review
- VISION - an open-source software for automated multi-dimensional image analysis of cellular biophysics.Journal of cell science · 2024Article
- Subphthalocyanine-flipper dyads for selective membrane staining.Physical chemistry chemical physics : PCCP · 2024Article
- Cell-Surface-Retained Peptide Additives for the Cytosolic Delivery of Functional Proteins.Journal of the American Chemical Society · 2023Article
- FluorogenicJACS Au · 2023Article
- Viscosity-Sensitive Membrane Dyes as Tools To Estimate the Crystalline Structure of Lipid Bilayers.Analytical chemistry · 2023Article
- Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent DithienothiopheneInternational journal of molecular sciences · 2023Article
- Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging.Biosensors · 2023Review
- The Dynamic Range of Acidity: Tracking Rules for the Unidirectional Penetration of Cellular Compartments.Chembiochem : a European journal of chemical biology · 2022Article
- Imidazo[1,5-Molecules (Basel, Switzerland) · 2022Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HydroFlippers are introduced as the first fluorescent membrane tension probes that report simultaneously on membrane compression and hydration. The probe design is centered around a sensing cycle that couples the mechanical planarization of twisted push-pull fluorophores with the dynamic covalent hydration of their exocyclic acceptor. In FLIM images of living cells, tension-induced deplanarization is reported as a decrease in fluorescence lifetime of the dehydrated mechanophore. Membrane hydration is reported as the ratio of the photon counts associated to the hydrated and dehydrated mechanophores in reconvoluted lifetime frequency histograms. Trends for tension-induced decompression and hydration of cellular membranes of interest (MOIs) covering plasma membrane, lysosomes, mitochondria, ER, and Golgi are found not to be the same. Tension-induced changes in mechanical compression are rather independent of the nature of the MOI, while the responsiveness to changes in hydration are highly dependent on the intrinsic order of the MOI. These results confirm the mechanical planarization of push-pull probes in the ground state as most robust mechanism to routinely image membrane tension in living cells, while the availability of simultaneous information on membrane hydration will open new perspectives in mechanobiology.
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Registered trials
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