ArticlePlant biotechnology journal2022
Expression of AtWRI1 and AtDGAT1 during soybean embryo development influences oil and carbohydrate metabolism.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 35 citations in OpenAlex.
- Natural variation inScience advances · 2026Article
- Single-Cell Transcriptomics Reveals the FA9-VAP Module Regulating Fatty Acid Accumulation in Soybean Seeds.Plant biotechnology journal · 2026Article
- Conserved regulatory modules and hub genes linking leaf transcriptomes to soybean seed weight.BMC plant biology · 2026Article
- Cell wall invertase improves grain nutrition via regulating sugar and hormone metabolism gene expression in transgenic soybean.Annals of botany · 2026Article
- Comparative Metabolomic Profiling of Resistant and SusceptiblePlants (Basel, Switzerland) · 2026Article
- Enhancing soybean (Frontiers in plant science · 2026Article
- Mapping carbon utilization pathways inmSystems · 2025Article
- Deciphering the role of monoacylglycerol lipases (MAGL) under abiotic stress and lipid metabolism in soybean (Glycine max L.).Plant biotechnology journal · 2025Article
- Enhanced seed oil content by overexpressing LPAAT and WRI1 genes in tobacco plant.Plant cell reports · 2025Article
- Metabolic engineering-induced transcriptome reprogramming of lipid biosynthesis enhances oil composition in oat.Plant biotechnology journal · 2024Article
- NgAP2a TargetsInternational journal of molecular sciences · 2024Article
- Single- and multiple-trait quantitative trait locus analyses for seed oil and protein contents of soybean populations with advanced breeding line background.Molecular breeding : new strategies in plant improvement · 2024Article
- Multi-gene engineering boosts oil content in rice grains.Plant communications · 2024Article
- QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean.Plants (Basel, Switzerland) · 2023Article
- Differential metabolic reprogramming in developing soybean embryos in response to nutritional conditions and abscisic acid.Plant molecular biology · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- Progress in understanding and improving oil content and quality in seeds.Frontiers in plant science · 2023Review
- Expression of AtWRI1 and AtDGAT1 during soybean embryo development influences oil and carbohydrate metabolism.Plant biotechnology journal · 2022Article
- A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry.Frontiers in plant science · 2022Article
- Genome wide association study to detect genetic regions related to isoflavone content in a mutant soybean population derived from radiation breeding.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo's responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and AtDGAT1 encodes an enzyme catalysing the final and rate-limiting step of triacylglycerides biosynthesis. The events expressing these genes in the embryo did not show an increase in total FA content, but they responded with changes in the oil and carbohydrate composition. Transcriptomic studies revealed a down-regulation of genes putatively encoding for oil body packaging proteins, and a strong induction of genes annotated as lipases and FA biosynthesis inhibitors. Novel putative AtWRI1 targets, presenting an AW-box in the upstream region of the genes, were identified by comparison with an event that harbours only AtWRI1. Lastly, targeted metabolomics analysis showed that carbon from sugar phosphates could be used for FA competing pathways, such as starch and cell wall polysaccharides, contributing to the restriction in oil accumulation. These results allowed the identification of key cellular processes that need to be considered to break the embryo's natural restriction to uncontrolled seed lipid increase.
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