Evidence map›Paper›PMID 35306474›Full record

ArticleThe Journal of pharmacology and experimental therapeutics2022

Neutrophil-Derived Myeloperoxidase and Hypochlorous Acid Critically Contribute to 20-Hydroxyeicosatetraenoic Acid Increases that Drive Postischemic Angiogenesis.

Juan A Azcona, Samantha Tang, Elizabeth Berry, Frank F Zhang, Radha Garvey, John R Falck, Michal Laniado Schwartzman, Tao Yi, Thomas M Jeitner, Austin M Guo

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of pharmacology and experimental therapeutics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Baicalin alleviates HScientific reports · 2025
    Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Juan A AzconaDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
Samantha TangDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
Elizabeth BerryDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
Frank F ZhangDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
Radha GarveyDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
John R FalckDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
Michal Laniado SchwartzmanDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
Tao YiDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
Thomas M JeitnerDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.).
Austin M GuoDepartment of Pharmacology (J.A.A., F.F.Z., M.L.S., A.M.G.), Department of Biochemistry and Molecular Biology (J.A.A., T.M.J., A.M.G.), Department of Pathology (S.T.), and Department of Physiology (E.B.), New York Medical College, Valhalla, New York; Department of Radiology, Weill Cornell Medicine, New York, New York (J.A.A., T.M.J.); Tufts University, Medford, Massachusetts (R.G.); University of Texas Southwestern Medical Center, Dallas, Texas (J.R.F.); and Department of Chemistry, Fudan University, Shanghai, PR China (T.Y.) austin_guo@nymc.edu.
Weill Cornell Medicine · USCornell University · USNew York Medical College · USThe University of Texas Southwestern Medical Center · USTufts University · US

Funding

Vasoregulatory Function of CYP2E1-Derived EicosanoidsP01HL034300 · NHLBI · NEW YORK MEDICAL COLLEGE · PI JIANG, HOULI · 1985 to 2015
$33.4M
Molecular Mechanisms of 20-HETE-mediated Vascular Dysfunction and HypertensionR01HL139793 · NHLBI · NEW YORK MEDICAL COLLEGE · PI SCHWARTZMAN, MICHAL LANIADO · 2018 to 2021
$2.7M
NHLBI NIH HHS P01 HL034300NHLBI NIH HHS R01 HL139793
6 · The paper itself

Abstract

Compensatory angiogenesis is an important adaptation for recovery from critical ischemia. We recently identified 20-hydroxyeicosatetraenoic acid (20-HETE) as a novel contributor of ischemia-induced angiogenesis. However, the precise mechanisms by which ischemia promotes 20-HETE increases that drive angiogenesis are unknown. This study aims to address the hypothesis that inflammatory neutrophil-derived myeloperoxidase (MPO) and hypochlorous acid (HOCl) critically contribute to 20-HETE increases leading to ischemic angiogenesis. Using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry, Laser Doppler Perfusion Imaging, and Microvascular Density analysis, we found that neutrophil depletion and MPO knockout mitigate angiogenesis and 20-HETE production in the gracilis muscles of mice subjected to hindlimb ischemia. Furthermore, we found MPO and HOCl to be elevated in these tissues postischemia as assessed by immunofluorescence microscopy and in vivo live imaging of HOCl. Next, we demonstrated that the additions of either HOCl or an enzymatic system for generating HOCl to endothelial cells increase the expression of CYP4A11 and its product, 20-HETE. Finally, pharmacological interference of hypoxia inducible factor (HIF) signaling results in ablation of HOCl-induced CYP4A11 transcript and significant reductions in CYP4A11 protein. Collectively, we conclude that neutrophil-derived MPO and its product HOCl activate HIF-1

Indexed as

Hypochlorous AcidPeroxidaseAnimalsEndothelial CellsHydroxyeicosatetraenoic AcidsIschemiaMiceNeovascularization, PathologicNeutrophils20-hydroxy-5,8,11,14-eicosatetraenoic acidHydroxyeicosatetraenoic AcidsHypochlorous AcidPeroxidase

Identifiers

PMID35306474
PMCPMC9190235
OpenAlexW4220970154

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.