Evidence map›Paper›PMID 35305575›Full record

SynthesisBMC genomics2022

DNA methylation changes in cord blood and the developmental origins of health and disease - a systematic review and replication study.

Loubna Akhabir, Randa Stringer, Dipika Desai, Piush J Mandhane, Meghan B Azad, Theo J Moraes, Padmaja Subbarao, Stuart E Turvey, Guillaume Paré, Sonia S Anand and 1 more

Open access · goldAbstract readSystematic Review
PubMed Full text at PMC Publisher
In one paragraph

Synthesis in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 1 country.

Loubna Akhabir *Population Health Research Institute, David Braley Cardiac, Vascular and Stroke Research Institute, 237 Barton Street East, Hamilton, ON, Canada.
Randa Stringer *Population Health Research Institute, David Braley Cardiac, Vascular and Stroke Research Institute, 237 Barton Street East, Hamilton, ON, Canada.
Dipika DesaiPopulation Health Research Institute, David Braley Cardiac, Vascular and Stroke Research Institute, 237 Barton Street East, Hamilton, ON, Canada.
Piush J MandhaneDepartment of Pediatrics, University of Alberta, Edmonton, AB, Canada.
Meghan B AzadDepartment of Pediatrics and Child Health, Children's Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, MB, Canada.
Theo J MoraesDepartment of Pediatrics, Hospital for Sick Children, ON, Toronto, Canada.
Padmaja SubbaraoDepartment of Medicine, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, ON, Canada.
Stuart E TurveyDepartment of Pediatrics, BC Children's Hospital, University of British Columbia, Vancouver, BC, Canada.
Guillaume ParéPopulation Health Research Institute, David Braley Cardiac, Vascular and Stroke Research Institute, 237 Barton Street East, Hamilton, ON, Canada.
Sonia S AnandPopulation Health Research Institute, David Braley Cardiac, Vascular and Stroke Research Institute, 237 Barton Street East, Hamilton, ON, Canada. anands@mcmaster.ca.ORCID http://orcid.org/0000-0003-3692-7441
NutriGen Alliance
Population Health Research Institute · CAHospital for Sick Children · CAChildren's Hospital Research Institute of Manitoba · CAUniversity of Alberta · CAUniversity of British Columbia · CAMcMaster University · CAImpact · CA

Funding

allergen allergencanada excellence research chairs, government of canada canada excellence research chairs, government of canadacanadian institute for health research 201301FH6canadian institute for health research AEC85761canadian institute for health research INC-109205heart and stroke foundation of canada NA7283
6 · The paper itself

Abstract

backgroundEnvironmental exposures in utero which modify DNA methylation may have a long-lasting impact on health and disease in offspring. We aimed to identify and replicate previously published genomic loci where DNA methylation changes are attributable to in utero exposures in the NutriGen birth cohort studies Alliance.

methodsWe reviewed the literature to identify differentially methylated sites of newborn DNA which are associated with the following five traits of interest maternal diabetes, pre-pregnancy body mass index (BMI), diet during pregnancy, smoking, and gestational age. We then attempted to replicate these published associations in the Canadian Healthy Infant Longitudinal Development (CHILD) and the South Asian birth cohort (START) cord blood epigenome-wide data.

resultsWe screened 68 full-text articles and identified a total of 17 cord blood epigenome-wide association studies (EWAS) of the traits of interest. Out of the 290 CpG sites reported, 19 were identified in more than one study; all of them associated with maternal smoking. In CHILD and START EWAS, thousands of sites associated with gestational age were identified and maintained significance after correction for multiple testing. In CHILD, there was differential methylation observed for 8 of the published maternal smoking sites. No other traits tested (i.e., folate levels, gestational diabetes, birthweight) replicated in the CHILD or START cohorts.

conclusionsMaternal smoking during pregnancy and gestational age are strongly associated with differential methylation in offspring cord blood, as assessed in the EWAS literature and our birth cohorts. There are a limited number of reported methylation sites associated in more than two independent studies related to pregnancy. Additional large studies of diverse populations with fine phenotyping are needed to produce robust epigenome-wide data in order to further elucidate the effect of intrauterine exposures on the infants' methylome.

Indexed as

DNA MethylationFetal BloodCanadaEpigenomeFemaleGenome-Wide Association StudyHumansInfant, NewbornPregnancyCHILDCord blood; methylation; EWAS; prenatalIn uteroNutrigenSTART

Identifiers

PMID35305575
PMCPMC8933946
OpenAlexW4221110947

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.