ArticleJournal of periodontal research2022
Ability of matrix metalloproteinase-8 biosensor, IFMA, and ELISA immunoassays to differentiate between periodontal health, gingivitis, and periodontitis.
Article in Journal of periodontal research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 36 citations in OpenAlex.
- Diagnostic value of MMP-8, IL-1β and IL-6 in peri-implantitis: a systematic review and meta-analysis.Oral and maxillofacial surgery · 2026Pooled it
- Assessment of salivary matrix metalloproteinase (MMP8) and activated salivary matrix metalloproteinase (aMMP8) in periodontitis patients: a systematic review and meta-analysis.Frontiers in oral health · 2025Pooled it
- Biochemical Fingerprint of Early Healing After Enamel Matrix Derivative Application Using a Flapless Approach: A Randomized Clinical Trial.International journal of molecular sciences · 2025Trial
- Periodontal Disease Diagnosis by a Chemically Etched Single-Mode Fiber-Optic Biosensor for Label-Free Detection of Matrix Metalloproteinase-8 (MMP-8).Biosensors · 2026Article
- Diagnostic accuracy and smoking-driven modulation of salivary MMP-8, MMP-9, and HIF-1α in stage III grade B/C periodontitis: a cross-sectional study.Clinical oral investigations · 2026Article
- Advanced Diagnostic Technologies and Molecular Biomarkers in Periodontitis: Systemic Health Implications and Translational Perspectives.Journal of clinical medicine · 2026Review
- Active Matrixmetalloproteinase-8 in Periodontal Diagnosis: A Scoping Review.Diagnostics (Basel, Switzerland) · 2025Review
- Diagnostic accuracy of aMMP-8 levels in oral biofluids for monitoring periodontitis in patients with metabolic syndrome.Clinical oral investigations · 2025Article
- Salivary and Microbiome Biomarkers in Periodontitis: Advances in Diagnosis and Therapy-A Narrative Review.Medicina (Kaunas, Lithuania) · 2025Review
- Article
- A Wearable Electrochemical Biosensor for Salivary Detection of Periodontal Inflammation Biomarkers: Molecularly Imprinted Polymer Sensor with Deep Learning Integration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Non-invasive dentistry method for clinical determination of bleeding time: evaluation in subjects with and without direct oral anticoagulant therapy.Thrombosis journal · 2025Article
- Article
- Evaluating salivary MMP-8 as a biomarker for periodontal diseases: A systematic review and meta-analysis.Heliyon · 2024Article
- Point-of-care diagnostic devices for periodontitis - current trends and urgent need.Sensors & diagnostics · 2024Review
- Impact of Inflammatory Markers and Senescence-Associated Secretory Phenotype in the Gingival Crevicular Fluid on the Outcomes of Periodontal Regeneration.International journal of molecular sciences · 2024Article
- Assessment of Salivary MMP-8 and IL-1β for the Diagnosis of Periodontal Diseases in Pakistani Population.European journal of dentistry · 2024Article
- Exploring the Efficacy of Novel Therapeutic Strategies for Periodontitis: A Literature Review.Life (Basel, Switzerland) · 2024Review
- A Comparative Analysis of Treatment-Related Changes in the Diagnostic Biomarker Active Metalloproteinase-8 Levels in Patients with Periodontitis.Diagnostics (Basel, Switzerland) · 2023Article
- Impact of local drug delivery and natural agents as new target strategies against periodontitis: new challenges for personalized therapeutic approach.Therapeutic advances in chronic disease · 2023Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
Abstract
objectiveThe aim of this study was to determine the diagnostic utility of an MMP-8 biosensor assay in differentiating periodontal health from gingivitis and periodontitis and compare it with an established time-resolved immunofluorescence assay (IFMA) and enzyme-linked immunosorbent assay (ELISA).
backgroundCurrently available antibody-based assays display a wide variability in their ability to accurately measure matrix metalloproteinase-8 (MMP-8) levels in saliva.
methodsSalivary MMP-8 levels were analyzed in 189 systemically healthy participants using an antibody-based biosensor prototype that operates using a surface acoustic wave technology and compared with IFMA and ELISA antibody assays. Participants were categorized into 3 groups: periodontal health (59), gingivitis (63), and periodontitis (67). A sub-population of participants (n = 20) with periodontitis received periodontal treatment and were monitored for 6 months.
resultsAll the assays demonstrated significantly higher salivary MMP-8 concentrations in participants with periodontitis versus gingivitis, periodontitis versus health, and gingivitis versus health (all p < .05). The biosensor data demonstrated significant correlations with IFMA (r = .354, p < .001) and ELISA (r = .681, p < .001). Significant reductions in salivary MMP-8 concentrations were detected by the biosensor (p = .030) and IFMA (p = .002) in participants with periodontitis 6 months after non-surgical periodontal treatment. IFMA had the best sensitivity (89.2%) for detecting periodontitis and gingivitis versus health and 96.6% for detecting periodontitis versus health and gingivitis. The biosensor had an AUC value of 0.81 and diagnostic accuracy of 74.2% for differentiating periodontitis and gingivitis from health; an AUC value of 0.86 and diagnostic accuracy of 82.8% for periodontitis versus health and gingivitis.
conclusionsThe biosensor, IFMA, and ELISA assays differentiated between periodontal health, gingivitis, and periodontitis based on salivary MMP-8 levels. Only the biosensor and, particularly, IFMA identified an effect of periodontal treatment in the participants with periodontitis. Our findings support the potential utility of salivary oral fluid aMMP-8-based point-of-care technology in the future of periodontal diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.