Evidence map›Paper›PMID 35304627›Full record

ArticleArchives of toxicology2022

Real-time monitoring of immediate drug response and adaptation upon repeated treatment in a microfluidic chip system.

Anastasiia Zuieva, Suzan Can, Franziska Boelke, Stefanie Reuter, Sebastian Schattscheider, Elfi Töpfer, Anika Westphal, Ralf Mrowka, Stefan Wölfl

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of toxicology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Anastasiia ZuievaInstitute of Pharmacy and Molecular Biotechnology, Pharmaceutical Biology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany.
Suzan CanInstitute of Pharmacy and Molecular Biotechnology, Pharmaceutical Biology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany.
Franziska BoelkeMicrofluidic ChipShop GmbH, Jena, Germany, Stockholmer Str. 20, 07747, Jena, Germany.
Stefanie ReuterExperimentelle Nephrologie, KIM III, 12 Universitätsklinikum Jena, Stockholmer Str. 20, 07747, Jena, Germany.
Sebastian SchattscheiderMicrofluidic ChipShop GmbH, Jena, Germany, Stockholmer Str. 20, 07747, Jena, Germany.
Elfi TöpferMicrofluidic ChipShop GmbH, Jena, Germany, Stockholmer Str. 20, 07747, Jena, Germany.
Anika WestphalExperimentelle Nephrologie, KIM III, 12 Universitätsklinikum Jena, Stockholmer Str. 20, 07747, Jena, Germany.
Ralf MrowkaExperimentelle Nephrologie, KIM III, 12 Universitätsklinikum Jena, Stockholmer Str. 20, 07747, Jena, Germany.
Stefan WölflInstitute of Pharmacy and Molecular Biotechnology, Pharmaceutical Biology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany. wolfl@uni-hd.de.ORCID 0000-0002-9977-174X

Funding

Bundesministerium für Bildung und Forschung 01EK1612ABundesministerium für Bildung und Forschung 01EK1612BBundesministerium für Bildung und Forschung 01EK1612C
6 · The paper itself

Abstract

Microfluidic tissue culture and organ-on-a-chip models provide efficient tools for drug testing in vivo and are considered to become the basis of in vitro test systems to analyze drug response, drug interactions and toxicity to complement and reduce animal testing. A major limitation is the efficient recording of drug action. Here we present an efficient experimental setup that allows long-term cultivation of cells in a microfluidic system in combination with continuous recording of luciferase reporter gene expression. The system combines a sensitive cooled luminescence camera system in combination with a custom build miniaturized incubation chamber. The setup allows to monitor time-dependent activation, but also the end of drug response. Repeated activation and recovery as well as varying durations of drug treatment periods can be monitored, and different modes of drug activity can be visualized.

Indexed as

Microfluidic Analytical TechniquesMicrofluidicsAnimalsLab-On-A-Chip DevicesLuciferaseMicrofluidic cultureOrgan-on-a-chip systemReal-time monitoring

Identifiers

PMID35304627
PMCPMC9013683

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.