SynthesisAnnals of clinical microbiology and antimicrobials2022
Detection performance of PCR for Legionella pneumophila in environmental samples: a systematic review and meta-analysis.
Synthesis in Annals of clinical microbiology and antimicrobials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- A Rapid and Sensitive CRISPR-Cas12a for the Detection ofPolish journal of microbiology · 2025Article
- Development of a PCR Assay for the Detection ofInfectious disease reports · 2025Article
- Sensitivity of Legionella pneumophila to phthalates and their substitutes.Scientific reports · 2023Article
- A Pilot Study forSensors (Basel, Switzerland) · 2023Article
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Authors and funding
11 authors.
Funding
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Abstract
backgroundLegionellosis remains a public health problem. The most common diagnostic method to detect Legionella pneumophila (L. pneumophila) is culture. Polymerase chain reaction (PCR) is a fast and accurate method for this detection in environmental samples.
methodsFour databases were searched for studies that evaluated the detection efficiency of PCR in L. pneumophila. The quality evaluation was conducted using Review Manager 5.3. We used Meta-DiSc 1.4 software and the Stata 15.0 software to create forest plots, a meta-regression, a bivariate boxplot and a Deeks' funnel plot.
resultsA total of 18 four-fold tables from 16 studies were analysed. The overall pooled sensitivity and specificity of PCR was 94% and 72%, respectively. The positive likelihood ratio (RLR) and negative likelihood ratio (NLR) was 2.73 and 0.12, respectively. The result of the diagnostic odds ratio (DOR) was 22.85 and the area under the curve (AUC) was 0.7884.
conclusionEstablishing a laboratory diagnostic tool for L. pneumophila detection is important for epidemiological studies. In this work, PCR demonstrated a promising diagnostic accuracy for L. pneumophila.
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