Evidence map›Paper›PMID 35303207›Full record

ArticleMarine biotechnology (New York, N.Y.)2022

Liposome-Encapsulated Rec8 and Dmrt1 Plasmids Induce Red-Spotted Grouper (Epinephelus akaara) Testis Maturation.

Xuzhuo Duan, Xianze Jia, Kaishan Liang, Fengqi Huang, Jinhong Shan, Huitao Chen, Xinhe Ruan, Lihua Li, Huihong Zhao, Qing Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. CpG site methylation regulates mouse Rec8 gene promoter activity.The Journal of reproduction and development · 2025
    Article
  2. Review
  3. Overview of Avian Sex Reversal.International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Xuzhuo DuanCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Xianze JiaCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Kaishan LiangCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Fengqi HuangCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Jinhong ShanCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Huitao ChenCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Xinhe RuanCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Lihua LiCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Huihong ZhaoCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China. zhaohh@scau.edu.cn.
Qing WangCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China. wangqing@scau.edu.cn.
South China Agricultural University · CNKey Laboratory of Guangdong Province · CN

Funding

Youth Fund Project of the National Natural Science Foundation of China 41806151
6 · The paper itself

Abstract

In fish, the maturity of gonads plays an important role in the development and reproduction of the population, and it also dictates the success of captive breeding. Therefore, finding ways to promote gonadal maturation is an important goal in aquaculture. In this study, we injected recombinant dmrt1 and rec8 overexpression plasmids packaged in liposomes into the immature testis of red-spotted grouper (Epinephelus akaara) and measured the expression of Dmrt1 and Rec8 protein in vivo. Gonadosomatic index (GSI) and gonadal histology analyses showed that the testis developed from the immature to the mature state within 7 days after plasmid injection. Additionally, the spermatozoa concentration and motility in plasmid-injected fish was the same as that of naturally mature fish. These results provided evidence that delivery of dmrt1 and rec8 expression plasmids into the testis via injection induced testis maturation in vivo.

Indexed as

BassAnimalsLiposomesMalePlasmidsSex DifferentiationTestisLiposomesDmrt1Epinephelus akaaraMatureRec8Testis

Identifiers

PMID35303207
OpenAlexW4220991313

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.