Evidence map›Paper›PMID 35301550›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2022

Deciphering the TLR transcriptome and downstream signaling pathway in cerebrospinal fluid in pediatric meningitis.

Manila Salaria, Sunit Singhi, Pratibha Singhi, Madhulika Sharma, Navdeep Mangat, Tanvi Bhatia, Ronny Wickstrom, Ritu Aggarwal

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Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Manila SalariaDepartment of Pediatrics, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Sunit SinghiDepartment of Pediatrics, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Pratibha SinghiDepartment of Pediatrics, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Madhulika SharmaDepartment of Immunopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Navdeep MangatDepartment of Immunopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Tanvi BhatiaDepartment of Immunopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Ronny WickstromNeuropediatric Unit, Astrid Lindgren's Children's Hospital, Karolinska University Hospital, Stockholm, Sweden.
Ritu AggarwalDepartment of Immunopathology, Post Graduate Institute of Medical Education and Research, Room No. 19, Research Block A, Fourth Floor, Chandigarh, 160012, India. ritu_immunopath@yahoo.co.in.ORCID http://orcid.org/0000-0001-5660-5031
Post Graduate Institute of Medical Education and Research · INMedanta The Medicity · INKarolinska University Hospital · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

OBJECTIVE/

designPediatric meningitis is characterized by a colossal inflammatory response to the pathogen in the central nervous system (CNS). This unabated inflammatory response persists even after the removal of the pathogen by antibiotics/steroids causing collateral damage to CNS tissue. Toll-like receptors (TLRs) are the key players in the recognition and elicitation of innate-immune response against bacterial/viral components in cerebrospinal fluid (CSF). Till date, the precise understanding of TLR-triggered inflammatory response in pediatric meningitis is lacking. The present study was designed to delineate the role of TLR transcriptome and downstream signaling pathways in CSF of pediatric meningitis.

methodsChildren in the age group of > 3 months to 12 years with pediatric meningitis were included. A total of 249 cases of pediatric meningitis (bacterial = 89, viral = 160) were included. In addition, 71 children who tested negative to the pathogen in CSF tap and did not have signs of infection clinically constituted the controls. RNA was extracted from the CSF samples of both cases and controls. The relative gene expression profile of 42 TLR signaling pathway genes was performed. For the analysis of secretory cytokines and chemokines in CSF, Luminex assay was performed.

resultsWe report global upregulation of TLR genes in patients with acute bacterial meningitis (ABM). The downstream signaling molecules were upregulated as well. The CSF of pediatric ABM patients revealed a predominant pro-inflammatory milieu marked by increased levels of pro-inflammatory cytokines. A significant correlation between poor clinical outcomes of patients and an increased expression of TLR/pro-inflammatory cytokine genes was observed.

conclusionOur findings provide support for future studies exploring TLR-based adjunct therapy to limit the neurological sequelae, owing to persistent inflammation in pediatric ABM patients.

Indexed as

Meningitis, BacterialToll-Like ReceptorsTranscriptomeChildChild, PreschoolCytokinesHumansSignal TransductionCytokinesToll-Like ReceptorsGene expressionLuminex assayMultiplex PCRPediatric meningitisTLR signaling

Identifiers

PMID35301550
OpenAlexW4221067424

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.