Evidence map›Paper›PMID 35301496›Full record

ArticleNature biotechnology2022

A programmable encapsulation system improves delivery of therapeutic bacteria in mice.

Tetsuhiro Harimoto, Jaeseung Hahn, Yu-Yu Chen, Jongwon Im, Joanna Zhang, Nicholas Hou, Fangda Li, Courtney Coker, Kelsey Gray, Nicole Harr and 6 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
125citing papers in PubMed, 2 pooled it
31.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

125 citing papers in PubMed, 2 syntheses or guidelines pooled it, 229 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Engineering an arginine-nitric oxide circuit inTranslational cancer research · 2026
    Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Living Microbial Drugs.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026
    Review
  12. Review
  13. Review
  14. Review
  15. Toward development of soil-derivedBiodesign research · 2026
    Article
  16. Review
  17. Review
  18. Review
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  20. Review

65 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 1 country.

Tetsuhiro Harimoto *Department of Biomedical Engineering, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0001-8859-7494
Jaeseung Hahn *Department of Biomedical Engineering, Columbia University, New York, NY, USA.
Yu-Yu ChenDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.
Jongwon ImDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0002-3660-2770
Joanna ZhangDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.
Nicholas HouDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.
Fangda LiDepartment of Microbiology and Immunology, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY, USA.
Courtney CokerDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.
Kelsey GrayDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0003-2940-1476
Nicole HarrDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.
Sreyan ChowdhuryDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.
Kelly PuDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.
Clare NimuraDepartment of Biomedical Engineering, Columbia University, New York, NY, USA.
Nicholas ArpaiaDepartment of Microbiology and Immunology, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0002-0657-0528
Kam W LeongDepartment of Biomedical Engineering, Columbia University, New York, NY, USA. kam.leong@columbia.edu.ORCID http://orcid.org/0000-0002-8133-4955
Tal DaninoDepartment of Biomedical Engineering, Columbia University, New York, NY, USA. tal.danino@columbia.edu.ORCID http://orcid.org/0000-0003-2713-7659
Columbia University · USColumbia University Irving Medical Center · US

Funding

Modulation of the tumor microenvironment with probiotic therapiesR01CA249160 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI ARPAIA, NICHOLAS, DANINO, TAL · 2020 to 2024
$3.0M
Engineering immunotherapeutic probiotics to mitigate irAEU01CA247573 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI ARPAIA, NICHOLAS, DANINO, TAL · 2020 to 2024
$2.9M
Engineering tumor-targeting bacteria to sense and deliver therapeuticsF99CA253756 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI HARIMOTO, TETSUHIRO · 2020 to 2021
$92k
NCI NIH HHS F99 CA253756NCI NIH HHS R01 CA249160NCI NIH HHS U01 CA247573
6 · The paper itself

Abstract

Living bacteria therapies have been proposed as an alternative approach to treating a broad array of cancers. In this study, we developed a genetically encoded microbial encapsulation system with tunable and dynamic expression of surface capsular polysaccharides that enhances systemic delivery. Based on a small RNA screen of capsular biosynthesis pathways, we constructed inducible synthetic gene circuits that regulate bacterial encapsulation in Escherichia coli Nissle 1917. These bacteria are capable of temporarily evading immune attack, whereas subsequent loss of encapsulation results in effective clearance in vivo. This dynamic delivery strategy enabled a ten-fold increase in maximum tolerated dose of bacteria and improved anti-tumor efficacy in murine models of cancer. Furthermore, in situ encapsulation increased the fraction of microbial translocation among mouse tumors, leading to efficacy in distal tumors. The programmable encapsulation system promises to enhance the therapeutic utility of living engineered bacteria for cancer.

Indexed as

Escherichia coliNeoplasmsAnimalsImmunotherapyMice

Identifiers

PMID35301496
PMCPMC9371971
OpenAlexW4220702606

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.