ArticleScientific reports2022
Detection of sex in adults and larvae of Leptinotarsa decemlineata on principle of copy number variation.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Tell Me How Much DNA You Have and I'll Tell You What Your Sex Is: Sex Determination by Flow Cytometry of Spiderlings ofEcology and evolution · 2026Article
- Copy Number Variation-Based Molecular Sexing ofbioRxiv : the preprint server for biology · 2025Article
- Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae).Scientific data · 2025Article
- Evidence for feminized genetic males in a flea beetle using newly identified X-linked markers.Ecology and evolution · 2024Article
- Investigation of genetic diversity and polyandry of Leptinotarsa decemlineata using X-linked microsatellite markers.Scientific reports · 2023Article
- Identification of sex-specific markers based on gene expression in adult Colorado potato beetles.Journal of insect science (Online) · 2023Article
- Bias in sex ratios and polyandry rate in reproduction of Leptinotarsa decemlineata.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The identification of sex in larvae of insects is usually challenging or even impossible, while in adults the sexual dimorphism is usually evident. Here, we used copy number analysis to develop a method of sex detection in Colorado potato beetle (Leptinotarsa decemlineata), which has an X0 sex determination system. The X linked gene LdVssc and autosomal gene LdUBE3B were identified as appropriate target and reference loci, respectively. The copy numbers (CNV) of LdVssc in males and females were estimated using standard droplet digital PCR (ddPCR) and real-time PCR (qPCR). With both methods, CNVs were bimodally distributed (BA
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