ArticleScientific reports2022
Effect of serum sample storage temperature on metabolomic and proteomic biomarkers.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Article
- Evaluating neonatal cord serum metabolome in association with adolescent cardiometabolic risk factors.Pediatric research · 2026Article
- Strategies for Monitoring Serum Protein Degradation With an Antibody Array-Based Technology.Journal of clinical laboratory analysis · 2026Article
- Article
- Serum Outperforms Plasma for Glypican-3 Quantification in Hepatocellular Carcinoma-A Prospective Comparative Study.Journal of clinical medicine · 2026Article
- Impact of long-term cryopreservation on serum proteome and metallome: Implications for Biobank quality control.PloS one · 2026Article
- Pitfalls of onco-metabolomics: impact of sample integrity on metabolomic investigations in more than 4500 human serum samples from ten different cohorts.Frontiers in molecular biosciences · 2026Article
- Sample Preparation for Multi-Omics Analysis: Considerations and Guidance for Identifying the Ideal Workflow.Proteomics · 2025Review
- A multivariate cell-based liquid biopsy for lung nodule risk stratification: Analytical validation and early clinical evaluation.The journal of liquid biopsy · 2025Article
- Ethical Biobanking in Extremely Preterm Infants: Lessons From the EPITOP Study.Acta paediatrica (Oslo, Norway : 1992) · 2025Article
- Cell Storage Conditions Impact Single-Cell Proteomic Landscapes.Journal of proteome research · 2025Article
- Cytokine-Based Insights into Bloodstream Infections and Bacterial Gram Typing in ICU COVID-19 Patients.Metabolites · 2025Article
- Berberine-inspired ionizable lipid for self-structure stabilization and brain targeting delivery of nucleic acid therapeutics.Nature communications · 2025Article
- Immunoaffinity Depletion of High-Abundance Proteins from Serum/Plasma for Proteomic Analysis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Association of over-the-counter mouthwash use with markers of nitric oxide metabolism, inflammation, and endothelial function-a cross-sectional study.Frontiers in oral health · 2025Article
- Early Mortality Prediction in Intensive Care Unit Patients Based on Serum Metabolomic Fingerprint.International journal of molecular sciences · 2024Article
- Evaluating protocols for reproducible targeted metabolomics by NMR.The Analyst · 2024Article
- A Framework for Quality Control in Quantitative Proteomics.Journal of proteome research · 2024Article
- Stable potassium isotope ratios in human blood serum towards biomarker development in Alzheimer's disease.Metallomics : integrated biometal science · 2024Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Prospective biomarker studies can be used to identify biomarkers predictive of disease onset. However, if serum biomarkers are measured years after their collection, the storage conditions might affect analyte concentrations. Few data exists concerning which metabolites and proteins are affected by storage at - 20 °C vs - 80 °C. Our objectives were to document analytes affected by storage of serum samples at - 20 °C vs - 80 °C, and to identify those indicative of the storage temperature. We utilized liquid chromatography tandem mass spectrometry and Luminex to quantify 300 analytes from serum samples of 16 Finnish individuals with type 1 diabetes, with split-aliquot samples stored at - 80 °C and - 20 °C for a median of 4.2 years. Results were validated in 315 Finnish and 916 Scottish individuals with type 1 diabetes, stored at - 20 °C and at - 80 °C, respectively. After quality control, we analysed 193 metabolites and proteins of which 120 were apparently unaffected and 15 clearly susceptible to storage at - 20 °C vs - 80 °C. Further, we identified serum glutamate/glutamine ratio greater than 0.20 as a biomarker of storage at - 20 °C vs - 80 °C. The results provide a catalogue of analytes unaffected and affected by storage at - 20 °C vs - 80 °C and biomarkers indicative of sub-optimal storage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.