ArticleGenome biology2022
CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells.
Article in Genome biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed.
- PATTY corrects open-chromatin bias for improved bulk and single-cell CUT&Tag profiling.Nature communications · 2026Article
- Many roads lead to a plant cistrome: mapping and interpreting transcription factor binding in plants.Genome biology · 2026Review
- Article
- Sequential RNA polymerase II activation drives human hematopoiesis.Cell reports · 2026Article
- A hitchhiker's guide to single-cell epigenomics: Methods and applications for cancer research.International journal of cancer · 2026Review
- Advances in scCUT&Tag and computational analysis for single-cell gene regulatory element mapping.Briefings in bioinformatics · 2026Review
- PATTY corrects open-chromatin bias for improved bulk and single-cell CUT&Tag profiling.Research square · 2025Article
- PATTY corrects open chromatin bias for improved bulk and single-cell CUT&Tag profiling.bioRxiv : the preprint server for biology · 2025Article
- Single-cell epigenomics and proteomics methods integrated in multiomics.Fundamental research · 2025Review
- Multi-omics based and AI-driven drug repositioning for epigenetic therapy in female malignancies.Journal of translational medicine · 2025Review
- DDX5 super-enhancer promotes vasculogenic mimicry formation and metastasis in nasopharyngeal carcinoma by enhancing ADAM10 transcription.Cell reports. Medicine · 2025Article
- Advances and applications in single-cell and spatial genomics.Science China. Life sciences · 2025Review
- Integrator complex subunit 12 knockout overcomes a transcriptional block to HIV latency reversal.eLife · 2025Article
- RNA polymerase II at histone genes predicts outcome in human cancer.Science (New York, N.Y.) · 2025Article
- Article
- Chromatin profiling for everyone: FFPE-CUTAC for the theory and practice of modern molecular biology.Frontiers in epigenetics and epigenomics · 2025Article
- RNA Polymerase II hypertranscription at histone genes in cancer FFPE samples.bioRxiv : the preprint server for biology · 2024Article
- Cut&tag: a powerful epigenetic tool for chromatin profiling.Epigenetics · 2024Review
- Single-cell multi-modal chromatin profiles revealing epigenetic regulations of cells in hepatocellular carcinoma.Clinical and translational medicine · 2024Article
- Progress in multifactorial single-cell chromatin profiling methods.Biochemical Society transactions · 2024Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ chromatin profiling in small samples and single cells. We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA polymerase II (Pol2 Serine-5 phosphate) and an antibody to repressive Polycomb domains (H3K27me3) followed by computational signal deconvolution to produce high-resolution maps of both the active and repressive regulomes in single cells. The ability to seamlessly map active promoters, enhancers, and repressive regulatory elements using a single workflow provides a complete regulome profiling strategy suitable for high-throughput single-cell platforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.