ReviewAnnual review of analytical chemistry (Palo Alto, Calif.)2022
Quantitative Stimulated Raman Scattering Microscopy: Promises and Pitfalls.
Review in Annual review of analytical chemistry (Palo Alto, Calif.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- Non-Invasively Quantitative Raman Imaging for Calculation of Lymphatic Transport Kinetics of Micelles in Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Illuminating aging with multimodal optical metabolic imaging.Science advances · 2026Review
- A new 4-atom linker enables PROTAC development and imaging.RSC chemical biology · 2026Article
- Let There be Light! Light as an Engine and Regulator in Synthetic Cells.Angewandte Chemie (International ed. in English) · 2026Review
- Article
- Optical imaging and spectroscopic characterization of subvisible particles in protein therapeutics.Advanced drug delivery reviews · 2026Review
- Mixed Domain IR-Hyper-Raman Four Wave Mixing Spectroscopy.The journal of physical chemistry letters · 2026Article
- Stimulated Raman Scattering Microscopy: Real-Time In-Situ Physical and Chemical Characterization of Reverse Osmosis Desalination Membrane Scaling.Environmental science & technology · 2025Article
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Artificial Intelligence-Powered Raman Spectroscopy through Open Science and FAIR Principles.ACS nano · 2025Review
- Sensitive Detection and Identification Method of Erythrocyte-like Cells upon Doxorubicin Induced Differentiation with Vibrational Techniques.Analytical chemistry · 2025Article
- Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy.Analytical chemistry · 2025Article
- Visible enhanced stimulated Raman scattering microscopy via frequency-doubling of picosecond pulses.Biomedical optics express · 2025Article
- Quantum theory of stimulated Raman scattering microscopy.Chemical physics reviews · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Principle of Stimulated Raman Scattering Microscopy: Emerging at High Spatiotemporal Limits.The journal of physical chemistry. C, Nanomaterials and interfaces · 2025Article
- SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction inbioRxiv : the preprint server for biology · 2025Article
- Absolute signal of stimulated Raman scattering microscopy: A quantum electrodynamics treatment.Science advances · 2024Article
- Endomicroscopic AI-driven morphochemical imaging and fs-laser ablation for selective tumor identification and selective tissue removal.Science advances · 2024Article
- Frequency-Domain Low-Wavenumber Hyperspectral Stimulated Raman Scattering Microscopy.Analytical chemistry · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Since its first demonstration, stimulated Raman scattering (SRS) microscopy has become a powerful chemical imaging tool that shows promise in numerous biological and biomedical applications. The spectroscopic capability of SRS enables identification and tracking of specific molecules or classes of molecules, often without labeling. SRS microscopy also has the hallmark advantage of signal strength that is directly proportional to molecular concentration, allowing for in situ quantitative analysis of chemical composition of heterogeneous samples with submicron spatial resolution and subminute temporal resolution. However, it is important to recognize that quantification through SRS microscopy requires assumptions regarding both system and sample. Such assumptions are often taken axiomatically, which may lead to erroneous conclusions without proper validation. In this review, we focus on the tacitly accepted, yet complex, quantitative aspect of SRS microscopy. We discuss the various approaches to quantitative analysis, examples of such approaches, challenges in different systems, and potential solutions. Through our examination of published literature, we conclude that a scrupulous approach to experimental design can further expand the powerful and incisive quantitative capabilities of SRS microscopy.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.