ArticlePlant physiology2022
Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- Trichomes in Plant Defence, Development and Metabolic Integration Under Environmental Stress.Plant, cell & environment · 2026Review
- Satellite DNA Editing Enables Multiplexed Chromosome Restructuring in Populus.Plant biotechnology journal · 2026Article
- A Dual-gRNA CRISPR/Cas9 System for Efficient Generation of Large Fragment Deletions in Poplar.Bio-protocol · 2026Article
- Conserved 4-coumarate 3-hydroxylase/ascorbate peroxidase bifunctionality coordinates lignin deposition and plant growth in Brachypodium and Populus.Plant physiology · 2026Article
- Effect of Genetic Ancestry on Ecologically Important Fitness Traits in HybridizingEvolutionary applications · 2026Article
- Streamlined multiplex CRISPR editing of receptor-like kinases in Populus via cell suspension transformation.Plant communications · 2025Article
- Towards an official gene nomenclature for Populus trichocarpa.Tree physiology · 2025Review
- Populus VariantDB v3.2 facilitates CRISPR and functional genomics research.Tree physiology · 2025Article
- Tonoplast sucrose transporter SUT4-dependent sugar partitioning modulates phenological transitions and reproductive success in poplar.The Plant journal : for cell and molecular biology · 2025Article
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- A compendium of nonredundant short polymerase III promoters for CRISPR applications.Plant physiology · 2025Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Crash-and-dash: a new era in tree genome editing.The New phytologist · 2025Article
- Hazelnut allergome overview and Cor a gRNAs identification.BMC plant biology · 2025Article
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- The transcriptional regulation of a putative hemicellulose gene, PtrPARVUS2 in poplar.Scientific reports · 2024Article
- Mapping QTLs for blight resistance and morpho-phenological traits in inter-species hybrid families of chestnut (Frontiers in plant science · 2024Article
- Multiplex Editing of theThe CRISPR journal · 2023Article
- CIDP: a multi-functional platform for designing CRISPR sgRNAs.Horticulture research · 2023Article
- Genome-Wide Identification and Analysis ofInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
17 authors at 4 institutions in 1 country.
Funding
Abstract
As the focus for CRISPR/Cas-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing for polygenic traits. A common approach for editing across multiple sites is to design one guide RNA (gRNA) per target; however, this complicates construct assembly and increases the possibility of off-target mutations. In this study, we utilized one gRNA to target MYB186, a known positive trichome regulator, as well as its paralogs MYB138 and MYB38 at a consensus site for mutagenesis in hybrid poplar (Populus tremula × P. alba INRA 717-1B4). Unexpected duplications of MYB186 and MYB138 resulted in eight alleles for the three targeted genes in the hybrid poplar. Deep sequencing and polymerase chain reaction analyses confirmed editing across all eight targets in nearly all of the resultant glabrous mutants, ranging from small indels to large genomic dropouts, with no off-target activity detected at four potential sites. This highlights the effectiveness of a single gRNA targeting conserved exonic regions for multiplex editing. Additionally, cuticular wax and whole-leaf analyses showed a complete absence of triterpenes in the trichomeless mutants, hinting at a previously undescribed role for the nonglandular trichomes of poplar.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.