ArticleScience (New York, N.Y.)2022
Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer.
Article in Science (New York, N.Y.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 111 citations in OpenAlex.
- Membrane properties modulate methane oxidation by particulate methane monooxygenase.The Journal of biological chemistry · 2026Article
- A Molecular Analogue of Cu/ZSM‑5 Catalyzing the Monooxygenation of Hydrocarbons via a Mono μ‑Oxo Dicopper Species.ACS omega · 2026Article
- Ammonia monooxygenase: a work in progress.Chemical science · 2026Review
- High-Valent Late Transition Metal-Oxo Complexes: Breaking Boundaries at the Oxo Wall and Beyond.Chemical reviews · 2026Review
- Unsymmetric bimetallic centres in metalloproteins and synthetic models.Nature reviews. Chemistry · 2026Review
- Structural insights into the catalytic mechanism of ammonia monooxygenase.Nature communications · 2025Article
- Structural and electronic modulations of lytic polysaccharide monooxygenase (LPMO) upon chitin binding: insights from X-ray spectroscopy.Chemical science · 2025Article
- Critical evaluation of three cryo-EM structures of particulate methane monooxygenase by quantum refinement.Acta crystallographica. Section D, Structural biology · 2025Article
- Solar-hybrid biocatalyst for methane hydroxylation to methanol.Nature communications · 2025Article
- Potential for aerobic hydrocarbon oxidation in archaea.Nature communications · 2025Article
- Histidine-Copper Site Variability in UiO-66: Monitoring Synthetic Intricacy with EPR Spectroscopy.Chemistry of materials : a publication of the American Chemical Society · 2025Article
- Advances in native cell membrane nanoparticles system.Current opinion in structural biology · 2025Review
- A Molecular Mechanism for the Reduction of the Cu Site in Lytic Polysaccharide Monooxygenases by Phenol Reductants.JACS Au · 2025Article
- Recent Advances in Bioinspired Cu-Directed C-H Hydroxylation Reactions.Accounts of chemical research · 2025Article
- Regioselective Hydration of Terpenes with Cofactor-Independent Carotenoid 1,2-Hydratase.Angewandte Chemie (International ed. in English) · 2025Article
- Harnessing the potential of microbial methane utilization for chasing sustainability.Current opinion in biotechnology · 2025Review
- Deciphering the language of mingling lipids and proteins.Current opinion in structural biology · 2025Review
- Mechanism of OACS central science · 2025Article
- Controlling outer-sphere solvent reorganization energy to turn on or off the function of artificial metalloenzymes.Nature communications · 2025Article
- Phylogenetic and Functional Diversity of Soluble Di-Iron Monooxygenases.Environmental microbiology · 2025Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo-electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.