ArticleActa parasitologica2022
A Convenient and Sensitive kDNA-PCR for Screening of Leishmania infantum Latent Infection Among Blood Donors in a Highly Endemic Focus, Northwestern Iran.
Article in Acta parasitologica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 10 citations in OpenAlex.
- Autochthonous Leishmaniasis in the United States of America.Microorganisms · 2025Review
- A one-step multiplex qPCR assay for simultaneous identification and quantification of Leishmania martiniquensis and Leishmania orientalis/Leishmania chancei and detection and quantification of trypanosomatids in clinical samples.Parasite (Paris, France) · 2025Article
- Recent advances in Phlebotomine sand fly research: a review based on studies presented at ISOPS XI.Parasite (Paris, France) · 2025Review
- Asymptomatic Leishmania Infection among Blood Donors in a Southern Province of Thailand.The American journal of tropical medicine and hygiene · 2024Article
- Prevalence of Leishmaniasis among Blood Donors: A Systematic Review and Meta-Analysis.Diseases (Basel, Switzerland) · 2024Review
- Asymptomatic Leishmania infantum infection in dogs and dog owners in an endemic area in southeast France.Parasite (Paris, France) · 2024Article
- Detection of asymptomatic Leishmania infection in blood donors at two blood banks in Ethiopia.PLoS neglected tropical diseases · 2023Article
- Molecular Detection of Neglected Tropical Diseases: The Case for Automated Near-Point-of-Care Diagnosis of Leishmaniasis.The American journal of tropical medicine and hygiene · 2023Article
- Common methods in mitochondrial research (Review).International journal of molecular medicine · 2022Article
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundRecent global evidences showed that asymptomatic blood donor carriers of Leishmania infection will appear as a threat for blood transfusions recipients in endemic areas. As yet, there is no appropriate diagnostic procedure for detecting infection of blood donors in blood banks. SUBJECTS AND
methodsThe present study was aimed to apply various current diagnostic tests among blood donors in an endemic area of visceral leishmaniasis (VL), Ardabil Province, northwestern Iran. Blood samples were gathered from 860 blood donors in endemic areas of the province between 2017 and 2018, at eight blood donation centers. These samples was assessed using microculture, serological (DAT and rK39-ICT) and molecular based (conventional kDNA-PCR and HRM-PCR) tests.
resultsOf 860 eligible donors, 24 (2.8%) were seropositive for VL by DAT, and 388 (45%) were positive by kDNA-PCR. Moreover, 19 (19/860) were positive for both of them. Out of 19 subjects, 5.3% (1/19) was positive by rK39-ICT, 10.5% (2/19), and 79% (15/19) were detected positive in microculture and HRM-PCR methods, respectively. Nineteen donors were followed up for 2 years, of which 16 (84.2%) had a serological conversion, and 4 (21%) were positive by kDNA-PCR. The sensitivity of kDNA-PCR, and HRM-PCR procedures in detecting Leishmania parasite was found to be 98.7%, and 79%, respectively.
conclusionsOur findings justify the use of kDNA-PCR as a convenient and sensitive tool for screening subjects with leishmanial latent infection in blood banks at least in endemic regions. In these areas, however, a PCR-based test should be used to validate Leishmania infection among seropositive donors.
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