ArticlePLoS pathogens2022
Differential viral RNA methylation contributes to pathogen blocking in Wolbachia-colonized arthropods.
Article in PLoS pathogens, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Three decades of analyzing Wolbachia bacterial endosymbionts in arthropods: Trends and gaps.Parasitology research · 2026Review
- Nanopore direct RNA sequencing (DRS) of MS2 bacteriophages in E. coli throughout its life cycles reveals a complex transcriptional activity to control and maintain its growth.Virology journal · 2026Article
- The inhibition mechanism ofFrontiers in microbiology · 2026Review
- Detection and genetic diversity of Wolbachia and its associated prophage WO in mosquito populations from Ethiopia.Scientific reports · 2025Article
- The intracellular symbiont Wolbachia alters Drosophila development and metabolism to buffer against nutritional stress.PLoS genetics · 2025Article
- Pseudouridine synthases are proviral factors for Sindbis virus in insect and mammalian cells.mBio · 2025Article
- Wolbachia elevates host methyltransferase expression and alters the mBMC microbiology · 2025Article
- The infectivity of virus particles from Wolbachia-infected Drosophila.BMC microbiology · 2025Article
- Review
- 2'-O-methyltransferase-deficient yellow fever virus: Restricted replication in the midgut and secondary tissues of Aedes aegypti mosquitoes severely limits dissemination.PLoS pathogens · 2024Article
- Recent insights into NCurrent opinion in genetics & development · 2024Review
- AntiviralmBio · 2024Article
- Article
- Common analysis of direct RNA sequencinG CUrrently leads to misidentification of mLife science alliance · 2024Article
- Evidence of Differences in Cellular Regulation ofViruses · 2024Article
- Host Derivation of Sindbis Virus Influences Mammalian Type I Interferon Response to Infection.Viruses · 2023Article
- Article
- The regulation of antiviral innate immunity through non-mFrontiers in immunology · 2023Review
- Host-Dependent Modifications of Packaged Alphavirus Genomic RNA Influence Virus Replication in Mammalian Cells.Viruses · 2022Article
- Transcriptional response ofThe Journal of general virology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Arthropod endosymbiont Wolbachia pipientis is part of a global biocontrol strategy to reduce the replication of mosquito-borne RNA viruses such as alphaviruses. We previously demonstrated the importance of a host cytosine methyltransferase, DNMT2, in Drosophila and viral RNA as a cellular target during pathogen-blocking. Here we report a role for DNMT2 in Wolbachia-induced alphavirus inhibition in Aedes species. Expression of DNMT2 in mosquito tissues, including the salivary glands, is elevated upon virus infection. Notably, this is suppressed in Wolbachia-colonized animals, coincident with reduced virus replication and decreased infectivity of progeny virus. Ectopic expression of DNMT2 in cultured Aedes cells is proviral, increasing progeny virus infectivity, and this effect of DNMT2 on virus replication and infectivity is dependent on its methyltransferase activity. Finally, examining the effects of Wolbachia on modifications of viral RNA by LC-MS show a decrease in the amount of 5-methylcytosine modification consistent with the down-regulation of DNMT2 in Wolbachia colonized mosquito cells and animals. Collectively, our findings support the conclusion that disruption of 5-methylcytosine modification of viral RNA is a vital mechanism operative in pathogen blocking. These data also emphasize the essential role of epitranscriptomic modifications in regulating fundamental alphavirus replication and transmission processes.
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