ReviewThe Journal of physiology2022
Advancing respiratory-cardiovascular physiology with the working heart-brainstem preparation over 25 years.
Review in The Journal of physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- MERTK-related engulfment of norepinephrinergic neuronal presynaptic membrane by astrocytes following acute TBI and potentiation effect of peripheral nerve modulation.Annals of medicine · 2026Article
- Modeling synaptic interactions between mammalian breathing and swallowing central pattern generators.Biological cybernetics · 2026Article
- Amino acid modulation of the carotid body selectively modulates peripheral chemoreceptor respiratory reflex.The Journal of physiology · 2026Article
- Exacerbated ATP transmission in the carotid body is linked to glomus cell expansion in spontaneously hypertensive rats.The Journal of physiology · 2026Article
- Vitamin B6 (Pyridoxal 5' Phosphate) antagonises carotid body P2X3 receptors in hypertension.Cardiovascular research · 2026Article
- Vasopressin Contributes to Respiratory and Cardiovascular Regulation in Spontaneously Hypertensive and Normotensive Rats.Journal of clinical medicine · 2025Article
- Oxytocin modulates respiratory heart rate variability through a hypothalamus-brainstem-heart neuronal pathway.Nature neuroscience · 2025Article
- Article
- Single-nucleus and spatial signatures of the brainstem in normal brain and mild traumatic brain injury in male mice.Nature communications · 2025Article
- Spatio-temporal segregation between sensory relay and swallowing pre-motor population activities by optical imaging in the rat nucleus of the solitary tract.Pflugers Archiv : European journal of physiology · 2025Article
- Inference technique for the synaptic conductances in rhythmically active networks and application to respiratory central pattern generation circuits.bioRxiv : the preprint server for biology · 2025Article
- Asymmetric neuromodulation in the respiratory network contributes to rhythm and pattern generation.Frontiers in neural circuits · 2025Article
- Spinal Afferent Innervation From Left Dorsal Root Ganglia in the Flat-Mounts of Whole Atria of Rats: Anterograde Tracing.The Journal of comparative neurology · 2024Article
- Vagal nerve stimulation in myocardial ischemia/reperfusion injury: from bench to bedside.Bioelectronic medicine · 2024Review
- Reduced Stroke Volume and Brain Perfusion Drive Postural Hyperventilation in Postural Orthostatic Tachycardia Syndrome.JACC. Basic to translational science · 2024Article
- Peritoneal sepsis caused by Escherichia coli triggers brainstem inflammation and alters the function of sympatho-respiratory control circuits.Journal of neuroinflammation · 2024Article
- Distribution and morphology of calcitonin gene-related peptide (CGRP) innervation in flat mounts of whole rat atria and ventricles.Autonomic neuroscience : basic & clinical · 2024Article
- Chronic intermittent hypoxia remodels catecholaminergic nerve innervation in mouse atria.The Journal of physiology · 2024Article
- Rapid coupling between vasculature and neurons through mechanosensitive channels in the olfactory lobe.Frontiers in human neuroscience · 2024Article
- Commonalities and differences in carotid body dysfunction in hypertension and heart failure.The Journal of physiology · 2023Article
Corrections and comments
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Authors and funding
20 authors.
Funding
Abstract
Twenty-five years ago, a new physiological preparation called the working heart-brainstem preparation (WHBP) was introduced with the claim it would provide a new platform allowing studies not possible before in cardiovascular, neuroendocrine, autonomic and respiratory research. Herein, we review some of the progress made with the WHBP, some advantages and disadvantages along with potential future applications, and provide photographs and technical drawings of all the customised equipment used for the preparation. Using mice or rats, the WHBP is an in situ experimental model that is perfused via an extracorporeal circuit benefitting from unprecedented surgical access, mechanical stability of the brain for whole cell recording and an uncompromised use of pharmacological agents akin to in vitro approaches. The preparation has revealed novel mechanistic insights into, for example, the generation of distinct respiratory rhythms, the neurogenesis of sympathetic activity, coupling between respiration and the heart and circulation, hypothalamic and spinal control mechanisms, and peripheral and central chemoreceptor mechanisms. Insights have been gleaned into diseases such as hypertension, heart failure and sleep apnoea. Findings from the in situ preparation have been ratified in conscious in vivo animals and when tested have translated to humans. We conclude by discussing potential future applications of the WHBP including two-photon imaging of peripheral and central nervous systems and adoption of pharmacogenetic tools that will improve our understanding of physiological mechanisms and reveal novel mechanisms that may guide new treatment strategies for cardiorespiratory diseases.
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