ArticlePlant physiology2022
An artificial host system enables the obligate parasite Cuscuta campestris to grow and reproduce in vitro.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Dodder seedlings benefit from apoplastic nutrition by inducing SWEET15-mediated unloading of sucrose from the host's phloem.The New phytologist · 2026Article
- Current understanding of the role of the cell wall in Cuscuta parasitism.Plant biology (Stuttgart, Germany) · 2025Review
- A unique phytochrome B gene in Cuscuta campestris and its responses during the initial stage of haustorium formation.Journal of plant research · 2025Article
- Involvement of MID1-COMPLEMENTING ACTIVITY 1 encoding a mechanosensitive ion channel in prehaustorium development of the stem parasitic plant Cuscuta campestris.Plant & cell physiology · 2025Article
- Agrobacterium-mediated Cuscuta campestris transformation as a tool for understanding plant-plant interactions.The New phytologist · 2025Article
- The early dodder gets the host: decoding the coiling patterns of Cuscuta campestris with automated image processing.Plant cell reports · 2024Article
- Gene complementation analysis indicates that parasitic dodder plants do not depend on the host FT protein for flowering.Plant communications · 2024Article
- A roadmap of haustorium morphogenesis in parasitic plants.Journal of experimental botany · 2023Article
- The plant vampire diaries: a historic perspective on Cuscuta research.Journal of experimental botany · 2023Review
- A host-free transcriptome for haustoriogenesis in Cuscuta campestris: Signature gene expression identifies markers of successive development stages.Physiologia plantarum · 2022Article
- Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cuscuta campestris is an obligate parasitic plant that requires a host to complete its life cycle. Parasite-host connections occur via a haustorium, a unique organ that acts as a bridge for the uptake of water, nutrients, and macromolecules. Research on Cuscuta is often complicated by host influences, but comparable systems for growing the parasite in the absence of a host do not exist. We developed an axenic method to grow C. campestris on an artificial host system (AHS). We evaluated the effects of nutrients and phytohormones on parasite haustoria development and growth. Haustorium morphology and gene expression were also characterized. The AHS consists of an inert, fibrous stick that mimics a host stem, wicking water and nutrients to the parasite. It enables C. campestris to exhibit a parasitic habit and develop through all stages of its life cycle, including production of new shoots and viable seeds. The phytohormones 1-naphthaleneacetic acid and 6-benzylaminopurine affect haustoria morphology and increase parasite fresh weight and biomass. Unigene expression in AHS haustoria reflects processes similar to those in haustoria on living host plants. The AHS is a methodological improvement for studying Cuscuta biology by avoiding specific host effects on the parasite and giving researchers full control of the parasite environment.
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