ArticleNucleic acids research2022
Retron reverse transcriptase termination and phage defense are dependent on host RNase H1.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 36 citations in OpenAlex.
- Detectrons convert transient RNA sequences into stable DNA barcodes for high-throughput analysis of RNA-dependent processes.Nature biotechnology · 2026Article
- Discovery and engineering of retrons for precise genome editing.Nature biotechnology · 2026Article
- Higher-order assembly of a type IX retron enables exploitation for designer antimicrobials.bioRxiv : the preprint server for biology · 2026Article
- Structural insights into the assembly and function of Retron Ec78 PtuAB.Communications biology · 2026Article
- Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering.Nature biotechnology · 2026Article
- Article
- Structure and mechanism of antiphage retron Eco2.Nature structural & molecular biology · 2026Article
- Structural mechanism of the Retron-Eco7 anti-phage defense system.Nature communications · 2025Article
- Phage nuclease-mediated defense activation of the bacterial Retron-Eco7 toxin-antitoxin system.Nucleic acids research · 2025Article
- New retron systems from environmental bacteria identify triggers of anti-phage defense and expand tools for genome editing.PLoS biology · 2025Article
- Phage therapy and its role in cancer treatment and control.Folia microbiologica · 2025Review
- Continuous multiplexed phage genome editing using recombitrons.Nature biotechnology · 2025Article
- An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaks.Nucleic acids research · 2025Article
- An experimental census of retrons for DNA production and genome editing.Nature biotechnology · 2025Article
- An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaks.bioRxiv : the preprint server for biology · 2025Article
- High throughput variant libraries and machine learning yield design rules for retron gene editors.Nucleic acids research · 2025Article
- Genetic Characterization of a Novel Retron Element Isolated from Vibrio mimicus.Microbiology and immunology · 2025Article
- Reducing competition between msd and genomic DNA improves retron editing efficiency.EMBO reports · 2024Article
- Simultaneous multi-site editing of individual genomes using retron arrays.Nature chemical biology · 2024Article
- High Throughput Variant Libraries and Machine Learning Yield Design Rules for Retron Gene Editors.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Retrons are bacterial retroelements that produce single-stranded, reverse-transcribed DNA (RT-DNA) that is a critical part of a newly discovered phage defense system. Short retron RT-DNAs are produced from larger, structured RNAs via a unique 2'-5' initiation and a mechanism for precise termination that is not yet understood. Interestingly, retron reverse transcriptases (RTs) typically lack an RNase H domain and, therefore, depend on endogenous RNase H1 to remove RNA templates from RT-DNA. We find evidence for an expanded role of RNase H1 in the mechanism of RT-DNA termination, beyond the mere removal of RNA from RT-DNA:RNA hybrids. We show that endogenous RNase H1 determines the termination point of the retron RT-DNA, with differing effects across retron subtypes, and that these effects can be recapitulated using a reduced, in vitro system. We exclude mechanisms of termination that rely on steric effects of RNase H1 or RNA secondary structure and, instead, propose a model in which the tertiary structure of the single-stranded RT-DNA and remaining RNA template results in termination. Finally, we show that this mechanism affects cellular function, as retron-based phage defense is weaker in the absence of RNase H1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.