Evidence map›Paper›PMID 35291301›Full record

ArticlebioRxiv : the preprint server for biology2024

SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion.

Jianchao Zhang, Andrew Kennedy, Daniel Macedo de Melo Jorge, Lijuan Xing, Whitney Reid, Sarah Bui, Joseph Joppich, Molly Rose, Sevval Ercan, Qiyi Tang and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 30 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Jianchao ZhangDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-3269-4639
Andrew KennedyDivision of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Daniel Macedo de Melo JorgeDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Lijuan XingDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Whitney ReidDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Sarah BuiDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Joseph JoppichDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Molly RoseDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Sevval ErcanDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Qiyi TangDepartment of Microbiology, Howard University College of Medicine, Washington, DC 20059, USA.
Andrew W TaiDivision of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Yanzhuang WangDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-1864-7094
University of Michigan · USHoward University · US

Funding

Dysregulation of p97/VCP disease mutants in IBM and FTLD-UR01NS102279 · NINDS · LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER · PI CHOU, TSUI-FEN · 2018 to 2022
$2.8M
Supplement: GOLGI BIOGENESIS AND FUNCTIONR35GM130331 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WANG, YANZHUANG · 2019 to 2023
$2.7M
How flaviviruses hijack a host transmembrane protein chaperone to promote viral infectionR01GM139823 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TAI, ANDREW W. · 2021 to 2024
$1.4M
HIV-1 Genomic RNA IntegrityR21AI152865 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KIDD, JEFFREY M, TAI, ANDREW W. · 2020 to 2021
$429k
NIAID NIH HHS R21 AI152865NIGMS NIH HHS R01 GM139823NIGMS NIH HHS R35 GM130331NINDS NIH HHS R01 NS102279
6 · The paper itself

Abstract

The COVID-19 pandemic is caused by SARS-CoV-2, an enveloped RNA virus. Despite extensive investigation, the molecular mechanisms for its assembly and secretion remain largely elusive. Here, we show that SARS-CoV-2 infection induces global alterations of the host endomembrane system, including dramatic Golgi fragmentation. SARS-CoV-2 virions are enriched in the fragmented Golgi. Disrupting Golgi function with small molecules strongly inhibits viral infection. Significantly, SARS-CoV-2 infection down-regulates GRASP55 but up-regulates TGN46 protein levels. Surprisingly, GRASP55 expression reduces both viral secretion and spike number on each virion, while GRASP55 depletion displays opposite effects. In contrast, TGN46 depletion only inhibits viral secretion without affecting spike incorporation into virions. TGN46 depletion and GRASP55 expression additively inhibit viral secretion, indicating that they act at different stages. Taken together, we show that SARS-CoV-2 alters Golgi structure and function to control viral assembly and secretion, highlighting the Golgi as a potential therapeutic target for blocking SARS-CoV-2 infection.

Indexed as

Golgi fragmentationGRASP55SARS-CoV-2TGN46traffickingviral assembly

Identifiers

PMID35291301
PMCPMC8923104
OpenAlexW4221038636

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.