ArticleApplied microbiology and biotechnology2022
Development of a p72 trimer-based colloidal gold strip for detection of antibodies against African swine fever virus.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 69 citations in OpenAlex.
- Development of an indirect ELISA based on a multi-epitope recombinant protein for antibody detection against African swine fever virus.Microbiology spectrum · 2026Article
- Identification and Characterization of a Novel Linear B-Cell Epitope Within the ASFV pB602L Protein for Serological Diagnosis.Microorganisms · 2026Article
- Rapid and visual dual-color lateral flow immunoassay for african swine fever virus antibody detection.Applied microbiology and biotechnology · 2026Article
- Review
- Development of a cost-effective serodiagnosis for African swine fever using solubility-enhanced recombinant p54, p30, and p72.Veterinary world · 2025Article
- A Mutant of Africa Swine Fever Virus Protein p72 Enhances Antibody Production and Regulates the Production of Cytokines.Viruses · 2025Article
- Progress in African Swine Fever Vector Vaccine Development.International journal of molecular sciences · 2025Review
- Article
- Development of a smartphone camera-based chemiluminescent lateral flow immunoassay for detecting African swine fever virus antibodies.Frontiers in microbiology · 2025Article
- The development of a lateral flow immunochromatographic test strip for measurement of specific IgA and IgG antibodies level against porcine epidemic diarrhea virus in pig milk.The veterinary quarterly · 2024Article
- Development of plate-type and tubular chemiluminescence immunoassay against African swine fever virus p72.Applied microbiology and biotechnology · 2024Article
- Nanobodies against African swine fever virus p72 and CD2v proteins as reagents for developing two cELISAs to detect viral antibodies.Virologica Sinica · 2024Article
- Epitope mapping and establishment of a blocking ELISA for mAb targeting the p72 protein of African swine fever virus.Applied microbiology and biotechnology · 2024Article
- Identification of Linear Epitopes in the C-Terminal Region of ASFV p72 Protein.Microorganisms · 2023Article
- A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response.Journal of nanobiotechnology · 2023Article
- Identification of monoclonal antibody targeting epitope on p72 trimeric spike of African swine fever virus.Virus genes · 2023Article
- A quadruple fluorescence quantitative PCR method for the identification of wild strains of african swine fever and gene-deficient strains.Virology journal · 2023Article
- Development of an enzyme-linked immunosorbent assay based on viral antigen capture by anti-spike glycoprotein monoclonal antibody for detecting immunoglobulin A antibodies against porcine epidemic diarrhea virus in milk.BMC veterinary research · 2023Article
- Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies.Frontiers in microbiology · 2023Article
- Development of an effective one-step double-antigen sandwich ELISA based on p72 to detect antibodies against African swine fever virus.Frontiers in veterinary science · 2023Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
African swine fever virus (ASFV) causes a highly contagious and often lethal swine viral disease, and leads to tremendous economic losses to the swine industry. Unfortunately, there are no vaccines and effective antiviral agents available to prevent and control ASFV outbreaks. Therefore, it is necessary to develop simple and rapid strategies to monitor ASFV-infected pigs to restrain its spread. In the current study, ASFV capsid protein p72 was expressed along with its chaperone pB602L to form trimers in human embryonic kidney 293 (HEK293) cells. The p72 trimers were subsequently labeled with colloidal gold to develop a immunochromatographic strip. The strip showed high specificity to ASFV-positive serum and no cross-reactivity to other swine virus positive sera. Importantly, the strip showed a higher sensitivity of detecting ASFV antibodies in both positive standard serum and clinical serum samples than a commercial enzyme-linked immunosorbent assay (ELISA) kit. Taken together, these results demonstrate the strip as a reliable diagnostic tool against ASFV infection, which will be appropriate for application in prevention and control of ASFV. KEY POINTS : • ASFV p72 trimers were successfully generated. • A colloidal gold strip was developed based on ASFV p72 trimers. • The strip is appropriate for detecting ASFV antibodies in the field.
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