Evidence map›Paper›PMID 35290642›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Purification and Characterization of Human DNA Ligase IIIα Complexes After Expression in Insect Cells.

Ishtiaque Rashid, Miaw-Sheue Tsai, Aleksandr Sverzhinsky, Aye Su Hlaing, Brian Shih, Aye C Thwin, Judy G Lin, Su S Maw, John M Pascal, Alan E Tomkinson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Ishtiaque Rashid *Departments of Internal Medicine, Molecular Genetics and Microbiology and the University of New Mexico Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM, USA.
Miaw-Sheue Tsai *Biological Systems and Engineering Division, Department of BioEngineering & BioMedical Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Aleksandr Sverzhinsky *Department of Biochemistry and Molecular Medicine, Université de Montréal, Montreal, QC, Canada.
Aye Su HlaingBiological Systems and Engineering Division, Department of BioEngineering & BioMedical Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Brian ShihBiological Systems and Engineering Division, Department of BioEngineering & BioMedical Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Aye C ThwinBiological Systems and Engineering Division, Department of BioEngineering & BioMedical Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Judy G LinBiological Systems and Engineering Division, Department of BioEngineering & BioMedical Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Su S MawBiological Systems and Engineering Division, Department of BioEngineering & BioMedical Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
John M PascalDepartment of Biochemistry and Molecular Medicine, Université de Montréal, Montreal, QC, Canada.
Alan E TomkinsonDepartments of Internal Medicine, Molecular Genetics and Microbiology and the University of New Mexico Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM, USA. atomkinson@salud.unm.edu.
Lawrence Berkeley National Laboratory · USUniversité de Montréal · CAUniversity of New Mexico · US

Funding

Transcription-Coupled & Replication-Associated Excision RepairP01CA092584 · NCI · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI WALTER J. CHAZIN · 2001 to 2026
$89.6M
Roles of LIG3 and XRCC1 genes in genome stability.R01ES012512 · NIEHS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Alan E Tomkinson · 2004 to 2026
$6.3M
NCI NIH HHS P01 CA092584NIEHS NIH HHS R01 ES012512
6 · The paper itself

Abstract

With improvements in biophysical approaches, there is growing interest in characterizing large, flexible multi-protein complexes. The use of recombinant baculoviruses to express heterologous genes in cultured insect cells has advantages for the expression of human protein complexes because of the ease of co-expressing multiple proteins in insect cells and the presence of a conserved post-translational machinery that introduces many of the same modifications found in human cells. Here we describe the preparation of recombinant baculoviruses expressing DNA ligase IIIα, XRCC1, and TDP1, their subsequent co-expression in cultured insect cells, the purification of complexes containing DNA ligase IIIα from insect cell lysates, and their characterization by multi-angle light scattering linked to size exclusion chromatography and negative stain electron microscopy.

Indexed as

DNA-Binding ProteinsDNA LigasesAnimalsDNA Ligase ATPHumansInsectaPoly-ADP-Ribose Binding ProteinsXenopus ProteinsX-ray Repair Cross Complementing Protein 1DNA-Binding ProteinsDNA Ligase ATPDNA LigasesPoly-ADP-Ribose Binding ProteinsXenopus ProteinsX-ray Repair Cross Complementing Protein 1XRCC1 protein, humanAffinity chromatographyBacmidBaculovirusInsect cellsIon exchange chromatographyMultiple angle light scatteringNegative stain electron microscopySize exclusion chromatography

Identifiers

PMID35290642
PMCPMC9278544
OpenAlexW4225515094

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.