Differential expression profiling of Oxycarenus laetus Kirby (Hemiptera: Lygaeidae) upon exposure to gossypol.

Shruthi Chalil Sureshan, Habeeb Shaik Mohideen, Mohandass Ramya

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

0.3field-weighted citation impact, top 46% of its field

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1 citing paper in PubMed, 4 citations in OpenAlex.

Tolerance to dietary linalool primarily involves co-expression of cytochrome P450s and cuticular proteins in Pagiophloeus tsushimanus (Coleoptera: Curculionidae) larvae using SMRT sequencing and RNA-seq.BMC genomics · 2023
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3 authors at 1 institution in 1 country.

Shruthi Chalil SureshanBioinformatics and Entomoinformatics Lab, Department of Genetic Engineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chengalpattu, Chennai, TN, 603203, India.

Habeeb Shaik MohideenBioinformatics and Entomoinformatics Lab, Department of Genetic Engineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chengalpattu, Chennai, TN, 603203, India. habeeb_skm@yahoo.co.in.ORCID http://orcid.org/0000-0003-4217-5063

Mohandass RamyaMolecular Genetics Laboratory, Department of Genetic Engineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chengalpattu, Chennai, TN, 603203, India.

SRM Institute of Science and Technology · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGossypium hirsutum seeds are rich in gossypol. In addition to its diverse beneficial properties, it is a known anti-fertility inducing agent in humans. Oxycarenus laetus feeds on the cottonseeds and yet its courtship, mating and reproduction is unaffected. METHODS AND

resultsIn this study, we performed a transcriptome profiling of O. laetus fed on Abutilon indicum (AB-no gossypol), G. hirsutum (GH-natural gossypol) and 1400 ppm commercial gossypol-soaked GH seeds (GHGO). Illumina NextSeq-500 paired-end 75 bp reads were generated and de novo assembled (48,214 genes) to identify the differentially expressed transcripts (DET) between the samples. Gene enrichment, KEGG pathway and cluster profiling of the DETs resulted in the identification of vital genes involved in the detoxification, pheromone biosynthesis, cuticle protein in the GHGO sample. Cyp4C1, Cyp6a13, Cyp6a14, Cyp4g15, Cyp4em8, Cyp303a1 were the detoxification related genes identified. Similarly, SDR dehydrogenase family 11 and fatty acid synthase in pheromone biosynthesis and cuticle proteins (RR1 and RR2) coding transcripts were found to be differentially expressed.

conclusionThis is the first study to report the expression of genes induced by gossypol in O. laetus. Based on the findings from the DET analysis, we conclude that the detoxification related genes of gossypol treated samples were affected.

Indexed as

GossypolHemipteraAnimalsGene Expression ProfilingGossypiumHumansPheromonesGossypolPheromonesBioinformaticsDetoxificationDifferentially expressed transcriptsGossypolOxycarenus laetusPheromone biosynthesis

Identifiers

PMID35290558

DOI10.1007/s11033-022-07322-3

OpenAlexW4220934427

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.