Evidence map›Paper›PMID 35289122›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022

Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ-on-a-Chip.

Girija Goyal, Pranav Prabhala, Gautam Mahajan, Bruce Bausk, Tal Gilboa, Liangxia Xie, Yunhao Zhai, Roey Lazarovits, Adam Mansour, Min Sun Kim and 11 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed
8.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 111 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
  5. iScience · 2026
    Article
  6. Review
  7. Lymphoid-Tissue-on-Chip Recapitulates Human Antibody Responses In Vitro.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Review
  13. Immunogenicity of Gene and Cell Therapies.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2026
    Review
  14. Article
  15. Review
  16. Article
  17. Review
  18. The Immunology of Transfusion Medicine: Past, Present, and Future.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
  19. Review
  20. Review

14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 3 institutions in 1 country.

Girija GoyalWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Pranav PrabhalaWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Gautam MahajanWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Bruce BauskWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Tal GilboaWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Liangxia XieWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Yunhao ZhaiWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Roey LazarovitsWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Adam MansourWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Min Sun KimWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Aditya PatilWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Danielle CurranWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Jaclyn M LongWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Sanjay SharmaWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Abidemi JunaidWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Limor CohenWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Thomas C FerranteWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Oren LevyWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Rachelle Prantil-BaunWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
David R WaltWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
Donald E IngberWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.ORCID 0000-0002-4319-6520
Harvard University · USBrigham and Women's Hospital · USBoston Children's Hospital · US

Funding

Lung-on-a-Chip Disease Models for Efficacy TestingUG3HL141797 · NHLBI · HARVARD UNIVERSITY · PI INGBER, DONALD E · 2017 to 2018
$2.8M
NHLBI NIH HHS UG3 HL141797NIH HHS UG3HL141797
6 · The paper itself

Abstract

Lymphoid follicles (LFs) are responsible for generation of adaptive immune responses in secondary lymphoid organs and form ectopically during chronic inflammation. A human model of ectopic LF formation will provide a tool to understand LF development and an alternative to non-human primates for preclinical evaluation of vaccines. Here, it is shown that primary human blood B- and T-lymphocytes autonomously assemble into ectopic LFs when cultured in a 3D extracellular matrix gel within one channel of a two-channel organ-on-a-chip microfluidic device. Superfusion via a parallel channel separated by a microporous membrane is required for LF formation and prevents lymphocyte autoactivation. These germinal center-like LFs contain B cells expressing Activation-Induced Cytidine Deaminase and exhibit plasma cell differentiation upon activation. To explore their utility for seasonal vaccine testing, autologous monocyte-derived dendritic cells are integrated into LF Chips. The human LF chips demonstrate improved antibody responses to split virion influenza vaccination compared to 2D cultures, which are enhanced by a squalene-in-water emulsion adjuvant, and this is accompanied by increases in LF size and number. When inoculated with commercial influenza vaccine, plasma cell formation and production of anti-hemagglutinin IgG are observed, as well as secretion of cytokines similar to vaccinated humans over clinically relevant timescales.

Indexed as

Influenza, HumanInfluenza VaccinesTertiary Lymphoid StructuresAnimalsAntibodies, ViralHumansLab-On-A-Chip DevicesSeasonsVaccinationAntibodies, ViralInfluenza Vaccinesantibodygerminal centerlymph nodeorgan chipvaccine

Identifiers

PMID35289122
PMCPMC9109055
OpenAlexW4221092793

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.