ArticleNature medicine2022
Pancreatic islet cryopreservation by vitrification achieves high viability, function, recovery and clinical scalability for transplantation.
Article in Nature medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Article
- Automated image analysis for intracellular ice detection in monolayers by freeze-substitution.Npj imaging · 2026Article
- Upconverting Nanoparticles for Bimodal Luminescence and Magnetic Resonance Imaging of Langerhans Islets.Small science · 2026Article
- Article
- Reconstitution of thymopoiesis via implantation of cryopreserved cultured thymus tissue into athymic recipients.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2026Article
- Cryopreservation of biological materials: applications and economic perspectives.In vitro cellular & developmental biology. Animal · 2026Review
- Advanced Rewarming Technologies for Cryopreservation.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Cryopreservation of ovarian tissue - what's known so far and future perspectives.Frontiers in reproductive health · 2026Review
- Vitrification and rapid rewarming of precision-cut liver slices for pharmacological and biomedical research.Bioengineering & translational medicine · 2026Article
- Comparative β-cell development: insights from Rodents, humans and induced pluripotent stem cell models.Stem cell research & therapy · 2025Review
- From cold chain to ambient: Benefits, risks, and evidence across cell therapy logistics.Molecular therapy. Methods & clinical development · 2025Review
- Islet dimension and its impact on transplant outcome: A systematic review.World journal of transplantation · 2025Article
- Stem cell therapies for diabetes.Nature medicine · 2025Review
- Dielectric properties of individual cryoprotective agents and cocktails VS55, M22, DP6 at subzero temperatures for cryopreservation.Scientific reports · 2025Article
- The need for an organoid manufacturing, preservation, and distribution center.Stem cells translational medicine · 2025Review
- Article
- Reviving hope: unlocking pancreatic islet immortality by optimizing a trehalose-based cryopreservation media and cell-penetrating peptide.Stem cell research & therapy · 2025Article
- Overcoming ice: cutting-edge materials and advanced strategies for effective cryopreservation of biosample.Journal of nanobiotechnology · 2025Review
- Islet Cell Replacement and Regeneration for Type 1 Diabetes: Current Developments and Future Prospects.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2025Review
- Vascular Microphysiological System for Investigating Endothelial Barrier Function During Organ Preservation and Reperfusion.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
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11 authors.
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Abstract
Pancreatic islet transplantation can cure diabetes but requires accessible, high-quality islets in sufficient quantities. Cryopreservation could solve islet supply chain challenges by enabling quality-controlled banking and pooling of donor islets. Unfortunately, cryopreservation has not succeeded in this objective, as it must simultaneously provide high recovery, viability, function and scalability. Here, we achieve this goal in mouse, porcine, human and human stem cell (SC)-derived beta cell (SC-beta) islets by comprehensive optimization of cryoprotectant agent (CPA) composition, CPA loading and unloading conditions and methods for vitrification and rewarming (VR). Post-VR islet viability, relative to control, was 90.5% for mouse, 92.1% for SC-beta, 87.2% for porcine and 87.4% for human islets, and it remained unchanged for at least 9 months of cryogenic storage. VR islets had normal macroscopic, microscopic, and ultrastructural morphology. Mitochondrial membrane potential and adenosine triphosphate (ATP) levels were slightly reduced, but all other measures of cellular respiration, including oxygen consumption rate (OCR) to produce ATP, were unchanged. VR islets had normal glucose-stimulated insulin secretion (GSIS) function in vitro and in vivo. Porcine and SC-beta islets made insulin in xenotransplant models, and mouse islets tested in a marginal mass syngeneic transplant model cured diabetes in 92% of recipients within 24-48 h after transplant. Excellent glycemic control was seen for 150 days. Finally, our approach processed 2,500 islets with >95% islets recovery at >89% post-thaw viability and can readily be scaled up for higher throughput. These results suggest that cryopreservation can now be used to supply needed islets for improved transplantation outcomes that cure diabetes.
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