ArticleScientific reports2022
Automated computation of nerve fibre inclinations from 3D polarised light imaging measurements of brain tissue.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Whole-slide mapping of tumor tissue fiber architecture via computational scattered light imaging.Biomedical optics express · 2026Article
- Measurement of the absolute value of the optical birefringence of myelin in primate brain tissue.Neurophotonics · 2026Article
- Alignment of histological and polarimetric large-scale imaging for brain tissue characterization.Journal of biomedical optics · 2025Article
- Impact of uniform illumination in widefield microscopy and mesoscopy.Scientific reports · 2025Article
- Polarimetric imaging of peripheral nerves: an intraoperative aid.Biomedical optics express · 2025Article
- Practical considerations for birefringence microscopy of myelin structure: Microscope design and tissue processing for effective imaging.Imaging neuroscience (Cambridge, Mass.) · 2024Article
- Self-supervised representation learning for nerve fiber distribution patterns in 3D-PLI.Imaging neuroscience (Cambridge, Mass.) · 2024Article
- Multiscale label-free imaging of myelin in human brain tissue with polarization-sensitive optical coherence tomography and birefringence microscopy.Biomedical optics express · 2023Article
- Using light and X-ray scattering to untangle complex neuronal orientations and validate diffusion MRI.eLife · 2023Article
- Widespread gray and white matter microstructural alterations in dual cognitive-motor deficit.Alzheimer's & dementia (Amsterdam, Netherlands)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The method 3D polarised light imaging (3D-PLI) measures the birefringence of histological brain sections to determine the spatial course of nerve fibres (myelinated axons). While the in-plane fibre directions can be determined with high accuracy, the computation of the out-of-plane fibre inclinations is more challenging because they are derived from the amplitude of the birefringence signals, which depends e.g. on the amount of nerve fibres. One possibility to improve the accuracy is to consider the average transmitted light intensity (transmittance weighting). The current procedure requires effortful manual adjustment of parameters and anatomical knowledge. Here, we introduce an automated, optimised computation of the fibre inclinations, allowing for a much faster, reproducible determination of fibre orientations in 3D-PLI. Depending on the degree of myelination, the algorithm uses different models (transmittance-weighted, unweighted, or a linear combination), allowing to account for regionally specific behaviour. As the algorithm is parallelised and GPU optimised, it can be applied to large data sets. Moreover, it only uses images from standard 3D-PLI measurements without tilting, and can therefore be applied to existing data sets from previous measurements. The functionality is demonstrated on unstained coronal and sagittal histological sections of vervet monkey and rat brains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.