ArticleScientific reports2022
Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Gene regulatory co-option drives birdsong neural circuit specialization.bioRxiv : the preprint server for biology · 2026Article
- Establishment of an immortalized Muscovy duck embryo fibroblast cell line and exploration of virus tropism.Poultry science · 2026Article
- Establishment and Characterization of a Chicken Myoblast Cell Line.International journal of molecular sciences · 2024Article
- Genomic loss of GPR108 disrupts AAV transduction in birds.bioRxiv : the preprint server for biology · 2024Article
- Analogies of human speech and bird song: From vocal learning behavior to its neural basis.Frontiers in psychology · 2023Review
- Establishment of the Primary Avian Gonadal Somatic Cell Lines for Cytogenetic Studies.Animals : an open access journal from MDPI · 2022Article
- Generation and characterization of genome-modified chondrocyte-like cells from the zebra finch cell line immortalized by c-MYC expression.Frontiers in zoology · 2022Article
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Authors and funding
7 authors.
Funding
Abstract
The zebra finch is one of the most commonly studied songbirds in biology, particularly in genomics, neuroscience and vocal communication. However, this species lacks a robust cell line for molecular biology research and reagent optimization. We generated a cell line, designated CFS414, from zebra finch embryonic fibroblasts using the SV40 large and small T antigens. This cell line demonstrates an improvement over previous songbird cell lines through continuous and density-independent growth, allowing for indefinite culture and monoclonal line derivation. Cytogenetic, genomic, and transcriptomic profiling established the provenance of this cell line and identified the expression of genes relevant to ongoing songbird research. Using this cell line, we disrupted endogenous gene sequences using S.aureus Cas9 and confirmed a stress-dependent localization response of a song system specialized gene, SAP30L. The utility of CFS414 cells enhances the comprehensive molecular potential of the zebra finch and validates cell immortalization strategies in a songbird species.
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