Evidence map›Paper›PMID 35286682›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Ion Channel-Based Reporters for cAMP Detection.

Thomas C Rich, Wenkuan Xin, Silas J Leavesley, C Michael Francis, Mark Taylor

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Thomas C RichDepartment of Pharmacology, University of South Alabama, Mobile, AL, USA. trich@southalabma.edu.
Wenkuan XinDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, USA.
Silas J LeavesleyDepartment of Pharmacology, University of South Alabama, Mobile, AL, USA.
C Michael FrancisCenter for Lung Biology, University of South Alabama, Mobile, AL, USA.
Mark TaylorDepartment of Physiology and Cell Biology, University of South Alabama, Mobile, AL, USA.
University of South Alabama · USUniversity of Tennessee Health Science Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the last 20 years tremendous progress has been made in the development of single cell cAMP sensors. Sensors are based upon cAMP binding proteins that have been modified to transduce cAMP concentrations into electrical or fluorescent readouts that can be readily detected using patch clamp amplifiers, photomultiplier tubes, or cameras. Here, we describe two complementary approaches for the detection and measurement of cAMP signals near the plasma membrane of cells using cyclic nucleotide (CNG) channel-based probes. These probes take advantage of the ability of CNG channels to transduce small changes in cAMP concentration into ionic flux through channel pores that can be readily detected by measuring Ca

Indexed as

Cyclic AMPCyclic Nucleotide-Gated Cation ChannelsCalciumCell MembraneSignal TransductionCalciumCyclic AMPCyclic Nucleotide-Gated Cation ChannelsAdenylyl cyclasecAMPCyclic nucleotide-gated channelGPCRPhosphodiesterase

Identifiers

PMID35286682
OpenAlexW4225558371

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.