Evidence map›Paper›PMID 35286675›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification.

Silas J Leavesley, Naga Annamdevula, Santina Johnson, D J Pleshinger, Thomas C Rich

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Silas J LeavesleyDepartment of Chemical and Biomolecular Engineering, University of South Alabama, Mobile, AL, USA. leavesley@southalabama.edu.
Naga AnnamdevulaDepartment of Pharmacology, University of South Alabama, Mobile, AL, USA.
Santina JohnsonDepartment of Pharmacology, University of South Alabama, Mobile, AL, USA.
D J PleshingerDepartment of Pharmacology, University of South Alabama, Mobile, AL, USA.
Thomas C RichDepartment of Pharmacology, University of South Alabama, Mobile, AL, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A variety of FRET probes have been developed to examine cAMP localization and dynamics in single cells. These probes offer a readily accessible approach to measure localized cAMP signals. However, given the low signal-to-noise ratio of most FRET probes and the dynamic nature of the intracellular environment, there have been marked limitations in the ability to use FRET probes to study localized signaling events within the same cell. Here, we outline a methodology to dissect kinetics of cAMP-mediated FRET signals in single cells using automated image analysis approaches. We additionally extend these approaches to the analysis of subcellular regions. These approaches offer a unique opportunity to assess localized cAMP kinetics in an unbiased, quantitative fashion.

Indexed as

Cyclic AMPFluorescence Resonance Energy TransferImage Processing, Computer-AssistedKineticsSignal-To-Noise RatioCyclic AMPCyclic nucleotideFörster resonance energy transferImage cytometryMicroscopy

Identifiers

PMID35286675

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.