Evidence map›Paper›PMID 35286670›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Imaging the cAMP Signaling Microdomain of the Primary Cilium Using Targeted FRET-Based Biosensors.

Danielle T Arena, Aldebaran M Hofer

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
  3. The Nociceptor Primary Cilium Contributes to Mechanical Nociceptive Threshold and Inflammatory and Neuropathic Pain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Danielle T ArenaVA Boston Healthcare System and the Department of Surgery, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
Aldebaran M HoferVA Boston Healthcare System and the Department of Surgery, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA. ahofer@rics.bwh.harvard.edu.
VA Boston Healthcare System · US

Funding

Probing the cAMP signaling microdomain of the primary ciliumR21DE025921 · NIDCR · HARVARD MEDICAL SCHOOL · PI HOFER, ALDEBARAN M · 2016 to 2017
$354k
BLRD VA I01 BX005124NIDCR NIH HHS R21 DE025921
6 · The paper itself

Abstract

Optical approaches have revolutionized our view of second messenger signaling in organelles, allowing precise time-resolved assessment of soluble signaling molecules in situ. Among the most challenging of subcellular signaling microdomains to assay is the primary cilium. A petite but visually arresting organelle, the primary cilium extends from the cell surface of most non-dividing cells. Recently, the concept of the primary cilium as an independent cAMP signaling organelle has attracted substantial interest. The cilium sequesters a very specific subset of ciliary cAMP-linked GPCRs in its membrane (e.g., 5-HT

Indexed as

Biosensing TechniquesCiliaCyclic AMPFluorescence Resonance Energy TransferSignal TransductionCyclic AMPcAMP signaling microdomainsFluorescent proteinsFRET biosensorsLive-cell ratio imagingPKAPrimary cilia

Identifiers

PMID35286670
OpenAlexW4226314311

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.