Evidence map›Paper›PMID 35286604›Full record

ArticleJournal of microbiology (Seoul, Korea)2022

Regulatory role of cysteines in (2R, 3R)-butanediol dehydrogenase BdhA of Bacillus velezensis strain GH1-13.

Yunhee Choi, Yong-Hak Kim

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of microbiology (Seoul, Korea), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Yunhee ChoiAgricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju, 55365, Republic of Korea.
Yong-Hak KimDepartment of Microbiology, Daegu Catholic University School of Medicine, Daegu, 42472, Republic of Korea. ykim@cu.ac.kr.
Catholic University of Daegu · KRRural Development Administration · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacillus velezensis strain GH1-13 contains a (2R,3R)-butanediol dehydrogenase (R-BDH) BdhA which converts acetoin to R-BD reversibly, however, little is known about its regulatory cysteine and biological significance. We performed site-directed mutation of three cysteines in BdhA. The C37S mutant had no enzyme activity and the C34S and C177S mutants differed from each other and wild type (WT). After zinc affinity chromatography, 1 mM ZnCl

Indexed as

Butylene GlycolsCysteineAlcohol OxidoreductasesBacillusHydrogen PeroxideAlcohol Oxidoreductasesbutanediol dehydrogenaseButylene GlycolsCysteineHydrogen Peroxide(2R, 3R)-butanediol dehydrogenaseBacillus velezensisPlant growth-promoting rhizobacteriumsite-directed mutagenesiszinc binding motif

Identifiers

PMID35286604
OpenAlexW4220683180

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.