ArticleNucleic acids research2022
A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- AtLYK4 is the major chitin receptor in Arabidopsis with N-glycosylation and ligand-interacting residues orchestrating chitin perception.Science China. Life sciences · 2026Article
- Versatile Applications of CRISPR-Based Programmable T-DNA Integration in Plants.Plant biotechnology journal · 2025Article
- Genome Editing by Grafting.International journal of molecular sciences · 2025Review
- Emerging trends in transgene-free crop development: insights into genome editing and its regulatory overview.Plant molecular biology · 2025Review
- Efforts to Downsize Base Editors for Clinical Applications.International journal of molecular sciences · 2025Review
- Knockout of phosphatidate phosphohydrolase genes confers broad-spectrum disease resistance in plants.Plant biotechnology journal · 2025Article
- A simple method to visualize pre-mRNA splicing with the naked eye using a genetically encoded visual splicing reporter.Plant physiology · 2024Article
- Engineering disease-resistant plants with alternative translation efficiency by switching uORF types through CRISPR.Science China. Life sciences · 2024Article
- Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors.Nucleic acids research · 2024Article
- Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture.Genome biology · 2024Article
- Modular cytosine base editing promotes epigenomic and genomic modifications.Nucleic acids research · 2024Article
- CRISPR base editor-based targeted random mutagenesis (BE-TRM) toolbox for directed evolution.BMB reports · 2024Review
- Efficient and heritable A-to-K base editing in rice and tomato.Horticulture research · 2024Article
- Split complementation of base editors to minimize off-target edits.Nature plants · 2023Article
- Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance.Journal of Zhejiang University. Science. B · 2023Review
- Recent advances in therapeutic CRISPR-Cas9 genome editing: mechanisms and applications.Molecular biomedicine · 2023Review
- Article
- Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform.Genome biology · 2023Article
- Ribo-uORF: a comprehensive data resource of upstream open reading frames (uORFs) based on ribosome profiling.Nucleic acids research · 2023Article
- Shoot-root signal circuit: Phytoremediation of heavy metal contaminated soil.Frontiers in plant science · 2023Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas-derived base editing tools empower efficient alteration of genomic cytosines or adenines associated with essential genetic traits in plants and animals. Diversified target sequences and customized editing products call for base editors with distinct features regarding the editing window and target scope. Here we developed a toolkit of plant base editors containing AID10, an engineered human AID cytosine deaminase. When fused to the N-terminus or C-terminus of the conventional Cas9 nickase (nSpCas9), AID10 exhibited a broad or narrow activity window at the protospacer adjacent motif (PAM)-distal and -proximal protospacer, respectively, while AID10 fused to both termini conferred an additive activity window. We further replaced nSpCas9 with orthogonal or PAM-relaxed Cas9 variants to widen target scopes. Moreover, we devised dual base editors with AID10 located adjacently or distally to the adenine deaminase ABE8e, leading to juxtaposed or spaced cytosine and adenine co-editing at the same target sequence in plant cells. Furthermore, we expanded the application of this toolkit in plants for tunable knockdown of protein-coding genes via creating upstream open reading frame and for loss-of-function analysis of non-coding genes, such as microRNA sponges. Collectively, this toolkit increases the functional diversity and versatility of base editors in basic and applied plant research.
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