ArticleApplied and environmental microbiology2022
Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.
Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Enhancing acetate-based biosurfactant production by Pseudomonas putida KT2440 through fermentation optimization.Microbial cell factories · 2026Article
- Multi-layered metabolic remodeling ofMetabolic engineering communications · 2026Article
- Field-validated microbial deodorant reduces hazardous gas exposure and improves air quality in rural household toilets.Journal of industrial microbiology & biotechnology · 2026Article
- Predicting Strain-Specific Metabolic Capabilities in the GenusComputational and structural biotechnology journal · 2026Article
- Biosensor-driven strain engineering reveals key cellular processes for maximizing isoprenol production inScience advances · 2025Article
- RB-TnSeq elucidates dicarboxylic-acid-specific catabolism in β-proteobacteria for improved plastic monomer upcycling.Applied and environmental microbiology · 2025Article
- Enhanced production of microbial levulinic acid through deletion of the levulinic acid transcriptional regulator (lvaR) in engineered Pseudomonas putida KT2440.Bioprocess and biosystems engineering · 2025Article
- EngineeringMetabolic engineering communications · 2025Article
- Upcycling of polyamides through chemical hydrolysis and engineered Pseudomonas putida.Nature microbiology · 2025Article
- Quorum Sensing Coordinates Carbon and Nitrogen Metabolism to Optimize Public Goods Production in Pseudomonas fluorescens 2P24.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Exploring the Metabolic Response of Pseudomonas putida to L-arginine.Advances in experimental medicine and biology · 2025Article
- Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis.Frontiers in plant science · 2025Article
- Into the metabolic wild: Unveiling hidden pathways of microbial metabolism.Microbial biotechnology · 2024Article
- Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture - interaction studies based on a multi-OMICs approach.Communications biology · 2024Article
- Machine learning analysis of RB-TnSeq fitness data predicts functional gene modules inmSystems · 2024Article
- Gene expression reprogramming ofMicrobiology (Reading, England) · 2024Article
- The phosphodiesterase RmcA contributes to the adaptation of Pseudomonas putida to l-arginine.FEMS microbiology letters · 2023Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas putida KT2440 has long been studied for its diverse and robust metabolisms, yet many genes and proteins imparting these growth capacities remain uncharacterized. Using pooled mutant fitness assays, we identified genes and proteins involved in the assimilation of 52 different nitrogen containing compounds. To assay amino acid biosynthesis, 19 amino acid drop-out conditions were also tested. From these 71 conditions, significant fitness phenotypes were elicited in 672 different genes including 100 transcriptional regulators and 112 transport-related proteins. We divide these conditions into 6 classes, and propose assimilatory pathways for the compounds based on this wealth of genetic data. To complement these data, we characterize the substrate range of three promiscuous aminotransferases relevant to metabolic engineering efforts
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