Evidence map›Paper›PMID 35285680›Full record

ArticleApplied and environmental microbiology2022

Required Gene Set for Autotrophic Growth of

Craig Woods, Christopher M Humphreys, Claudio Tomi-Andrino, Anne M Henstra, Michael Köpke, Sean D Simpson, Klaus Winzer, Nigel P Minton

Open access · hybridAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Gene essentiality in the solventogenicApplied and environmental microbiology · 2024
    Article
  6. Recent progress in engineeringSynthetic and systems biotechnology · 2024
    Review
  7. Review
  8. Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacteriumProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  9. Base editing enables duplex point mutagenesis inFrontiers in bioengineering and biotechnology · 2023
    Article
  10. Chromosomal engineering of inducible isopropanol- butanol-ethanol production inFrontiers in bioengineering and biotechnology · 2023
    Article
  11. Metabolic changes of the acetogenFrontiers in microbiology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Craig WoodsClostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.
Christopher M HumphreysClostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.
Claudio Tomi-AndrinoClostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.
Anne M HenstraClostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.
Michael KöpkeLanzaTech, Inc., Skokie, Illinois, USA.
Sean D SimpsonLanzaTech, Inc., Skokie, Illinois, USA.
Klaus WinzerClostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.ORCID 0000-0002-3956-8096
Nigel P MintonClostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottinghamgrid.4563.4, Nottingham, United Kingdom.ORCID 0000-0002-9277-1261
Engineering and Physical Sciences Research Council · GBAZ Technology (United States) · US

Funding

Biotechnology and Biological Sciences Research Council BB/K00283X/1Biotechnology and Biological Sciences Research Council BB/L013940/1Biotechnology and Biological Sciences Research Council BB/L502030/1
6 · The paper itself

Abstract

The majority of the genes present in bacterial genomes remain poorly characterized, with up to one-third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput technique that can help rectify this deficiency. The technology, however, can only be realistically applied to those species in which high rates of DNA transfer can be achieved. Here, we have developed a number of approaches that overcome this barrier in the autotrophic species Clostridium autoethanogenum by using a

Indexed as

Carbon MonoxideClostridiumAutotrophic ProcessesDNA Transposable ElementsGenome, BacterialMutagenesis, InsertionalCarbon MonoxideDNA Transposable ElementsacetogenchemoautotrophyClostridium autoethanogenumgene essentialityminimal genomeTn-SeqTraDIStransposonstransposon sequencingWood-Ljungdahl

Identifiers

PMID35285680
PMCPMC9004356
OpenAlexW4221127229

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.