ArticlePlant physiology2022
Inducible epigenome editing probes for the role of histone H3K4 methylation in Arabidopsis heat stress memory.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 61 citations in OpenAlex.
- The CRISPR-dCas9 epigenetic toolbox: prospects for modulating gene expression in climate-resilient crops.Planta · 2026Review
- Principles and mechanisms of plant acclimation to heat stress.Nature reviews. Molecular cell biology · 2026Review
- Cross-stress memory in plants: mobile RNAs, extracellular vesicles, and local-to-systemic signal integration.Plant molecular biology · 2026Review
- Histone demethylase CsJMJ12 enhances resistance of Navel orange to Penicillium digitatum via H3K27me3 demethylation.Journal of advanced research · 2026Article
- Dynamic regulatory mechanisms of histone methylation in plant development and environmental adaptation.Horticulture research · 2026Article
- Mechanisms and applications of epigenome editing in plants: current status, challenges and future perspectives.Functional & integrative genomics · 2025Review
- CRISPR targeting of H3K4me3 activates gene expression and unlocks centromere-proximal crossover recombination in Arabidopsis.Nature communications · 2025Article
- Promoter and domain swap analysis delineates heat stress memory-specific determinants of heat shock factor HSFA2.Plant physiology · 2025Article
- Exploring the untapped potential of single-cell and spatial omics in plant biology.The New phytologist · 2025Review
- CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025Review
- Priming thermotolerance: unlocking heat resilience for climate-smart crops.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- CRISPR/dCas9-targeted H3K27me3 demethylation at theiScience · 2025Article
- Fungal Endophytes: An Insight into Diversity, Stress Tolerance, Biocontrol and Plant Growth-Promoting Potentials.Current microbiology · 2025Review
- Functions and Mechanisms of Histone Modifications in Plants.Annual review of plant biology · 2025Review
- The generation of novel epialleles in plants: the prospective behind re-shaping the epigenome.Frontiers in plant science · 2025Review
- Strike at the root: exploring the transferability of heat stress tolerance in tomatoes by reciprocal grafting.Frontiers in plant science · 2025Article
- dCas-Based Tools to Visualize Chromatin or Modify Epigenetic Marks at Specific Plant Genomic Loci.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Modern Plant Breeding Techniques in Crop Improvement and Genetic Diversity: From Molecular Markers and Gene Editing to Artificial Intelligence-A Critical Review.Plants (Basel, Switzerland) · 2024Review
- Endophytic Fungi for Crops Adaptation to Abiotic Stresses.Microorganisms · 2024Review
- The Mediator kinase module enhances polymerase activity to regulate transcriptional memory after heat stress in Arabidopsis.The EMBO journal · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histone modifications play a crucial role in the integration of environmental signals to mediate gene expression outcomes. However, genetic and pharmacological interference often causes pleiotropic effects, creating the urgent need for methods that allow locus-specific manipulation of histone modifications, preferably in an inducible manner. Here, we report an inducible system for epigenome editing in Arabidopsis (Arabidopsis thaliana) using a heat-inducible dCas9 to target a JUMONJI (JMJ) histone H3 lysine 4 (H3K4) demethylase domain to a locus of interest. As a model locus, we target the ASCORBATE PEROXIDASE2 (APX2) gene that shows transcriptional memory after heat stress (HS), correlating with H3K4 hyper-methylation. We show that dCas9-JMJ is targeted in a HS-dependent manner to APX2 and that the HS-induced overaccumulation of H3K4 trimethylation (H3K4me3) decreases when dCas9-JMJ binds to the locus. This results in reduced HS-mediated transcriptional memory at the APX2 locus. Targeting an enzymatically inactive JMJ protein in an analogous manner affected transcriptional memory less than the active JMJ protein; however, we still observed a decrease in H3K4 methylation levels. Thus, the inducible targeting of dCas9-JMJ to APX2 was effective in reducing H3K4 methylation levels. As the effect was not fully dependent on enzyme activity of the eraser domain, the dCas9-JMJ fusion protein may act in part independently of its demethylase activity. This underlines the need for caution in the design and interpretation of epigenome editing studies. We expect our versatile inducible epigenome editing system to be especially useful for studying temporal dynamics of chromatin modifications.
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