Evidence map›Paper›PMID 35284960›Full record

ArticleArchives of gynecology and obstetrics2022

Circ_0062558 promotes growth, migration, and glutamine metabolism in triple-negative breast cancer by targeting the miR-876-3p/SLC1A5 axis.

Mengzhen Yuan, Jun Zhang, Yuxin He, Guangming Yi, Liwen Rong, Liangjian Zheng, Tingting Zhan, Congming Zhou

Abstract read
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In one paragraph

Article in Archives of gynecology and obstetrics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Targeting glutamine metabolism as a potential target for cancer treatment.Journal of experimental & clinical cancer research : CR · 2025
    Review
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  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Mengzhen YuanDepartment of Oncology, The Third People's Hospital of Chengdu, No.82, Qinglong Street, Qingyang District, Chengdu, 610031, Sichuan, China.
Jun ZhangDepartment of Oncology, The Third People's Hospital of Chengdu, No.82, Qinglong Street, Qingyang District, Chengdu, 610031, Sichuan, China.
Yuxin HeDepartment of Oncology, The Third People's Hospital of Chengdu, No.82, Qinglong Street, Qingyang District, Chengdu, 610031, Sichuan, China.
Guangming YiThe Third Hospital of Mianyang, Sichuan Mental Health Center, Mianyang, Sichuan, China.
Liwen RongDepartment of Oncology, The Third People's Hospital of Chengdu, No.82, Qinglong Street, Qingyang District, Chengdu, 610031, Sichuan, China.
Liangjian ZhengDepartment of Oncology, The Third People's Hospital of Chengdu, No.82, Qinglong Street, Qingyang District, Chengdu, 610031, Sichuan, China.
Tingting ZhanDepartment of Oncology, The Third People's Hospital of Chengdu, No.82, Qinglong Street, Qingyang District, Chengdu, 610031, Sichuan, China.
Congming ZhouDepartment of Oncology, The Third People's Hospital of Chengdu, No.82, Qinglong Street, Qingyang District, Chengdu, 610031, Sichuan, China. tumor9398@163.com.
Chengdu Third People's Hospital · CNMianyang Third People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCircular RNAs (circRNAs) have been reported to function as vital regulators in cancers, including triple-negative breast cancer (TNBC). This study aimed to explore the role of circ_0062558 in TNBC.

methodsThe real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to quantify the expressions of circ_0062558, microRNA-876-3p (miR-876-3p), and solute carrier family 1 (neutral amino acid transporter), member 5 (SLC1A5) in TNBC tissues and cells. 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazol-3-ium bromide (MTT), thymidine analog 5-ethynyl-2'-deoxyuridine (EdU), flow cytometry, wound healing, and Transwell assays were employed for cell phenotype analyses. Protein expression was tested by western blot analysis. Dual-luciferase reporter was used to confirm the association among circ_0062558, miR-876-3p, and SLC1A5 in TNBC. Xenograft experiments were performed to elucidate the function of circ_0062558 in vivo.

resultsTNBC tissues and cells showed the higher level of circ_0062558 when compared with control samples. Downregulation of circ_0062558 inhibited proliferation, migration, invasion, and glutamine metabolism, while enhanced apoptosis of TNBC cells, and silencing of circ_0062558 also inhibited the growth of tumor in vivo. MiR-876-3p was confirmed as a target of circ_0062558, and circ_0062558 knockdown repressed TNBC cell malignant behaviors by increasing miR-876-3p. Furthermore, miR-876-3p inhibited malignant behaviors of TNBC cells by down-regulating SLC1A5, a newly identified target of miR-876-3p.

conclusionCirc_0062558 promoted TNBC progression by enhancing proliferation, survival, migration, invasion, and glutamine metabolism via miR-876-3p/SLC1A5 axis, which was helpful for understanding the carcinogenic roles of circ_0062558.

Indexed as

Amino Acid Transport Systems, NeutralMicroRNAsTriple Negative Breast NeoplasmsAmino Acid Transport System ASCAnimalsBromidesCell Line, TumorCell MovementCell ProliferationGene Expression Regulation, NeoplasticGlutamineHumansMinor Histocompatibility AntigensRNA, CircularThymidineAmino Acid Transport System ASCAmino Acid Transport Systems, NeutralBromidesGlutamineMicroRNAsMinor Histocompatibility AntigensMIRN876 microRNA, humanRNA, CircularSLC1A5 protein, humanThymidineCirc_0062558CircRNAMiR-876-3pSLC1A5TNBC

Identifiers

PMID35284960
OpenAlexW4220843280

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.