Evidence map›Paper›PMID 35284843›Full record

ArticleSTAR protocols2022

A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system.

Mustapha Aouida, Dina Aljogol, Reem Ali, Dindial Ramotar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mustapha AouidaCollege of Health and Life Sciences, Division of Biological and Biomedical Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation, Doha 34110, Qatar.
Dina AljogolCollege of Health and Life Sciences, Division of Genomics and Precision Medicine, Hamad Bin Khalifa University, Education City, Qatar Foundation, Doha 34110, Qatar.
Reem AliCollege of Health and Life Sciences, Division of Biological and Biomedical Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation, Doha 34110, Qatar.
Dindial RamotarCollege of Health and Life Sciences, Division of Biological and Biomedical Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation, Doha 34110, Qatar.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we describe a protocol for human PRDX1 gene knockout cells using the CRISPR-Cas9 system. The protocol describes all the steps sequentially: (1) single-guide RNA design, cloning, and transfection; (2) gene editing evaluation by T7EI assay; (3) single-cell isolation; and (4) knockout verification to determine indels in one or both alleles by Sanger sequencing. This strategy is based on the efficiency of DNA editing, avoids antibiotic selection, and bypasses the need for cell sorting.

Indexed as

CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsGene EditingGene Knockout TechniquesHumansPeroxiredoxinsTransfectionPeroxiredoxinsPRDX1 protein, humanRNA, Guide, CRISPR-Cas SystemsBiotechnology and bioengineeringCell BiologyCell cultureCell isolationCRISPRMolecular Biology

Identifiers

PMID35284843
PMCPMC8904610

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.